ArticleThe American journal of pathology2000
High-throughput tissue microarray analysis of cyclin E gene amplification and overexpression in urinary bladder cancer.
Article in The American journal of pathology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 243 citations in OpenAlex.
- Assessing the significance of CCNE1 overexpression in triple negative breast cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026Article
- Diet quality, common genetic polymorphisms, and bladder cancer risk in a New England population-based study.European journal of nutrition · 2022Article
- A Network-guided Association Mapping Approach from DNA Methylation to Disease.Scientific reports · 2019Article
- Urinary metabolites for urological cancer detection: a review on the application of volatile organic compounds for cancers.American journal of clinical and experimental urology · 2019Review
- Cyclin E Overexpression Sensitizes Triple-Negative Breast Cancer to Wee1 Kinase Inhibition.Clinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Prognostic Values ofJournal of Cancer · 2018Article
- Comparative Bioinformatics Characteristic of Bladder Cancer Stage 2 from Stage 4 Expression Profile: A Network-Based Study.Galen medical journal · 2018Article
- Gene Expression Detection Assay for Cancer Clinical Use.Journal of Cancer · 2018Review
- Nuclear overexpression of the overexpressed in lung cancer 1 predicts worse prognosis in gastric adenocarcinoma.Oncotarget · 2017Article
- Bioinformatic identification of prognostic signature defined by copy number alteration and expression of CCNE1 in non-muscle invasive bladder cancer.Experimental & molecular medicine · 2017Article
- Tissue Microarray Analysis Applied to Bone Diagenesis.Scientific reports · 2017Article
- Transcription Factor KLF5 Binds a Cyclin E1 Polymorphic Intronic Enhancer to Confer Increased Bladder Cancer Risk.Molecular cancer research : MCR · 2016Article
- Pathway analysis of bladder cancer genome-wide association study identifies novel pathways involved in bladder cancer development.Genes & cancer · 2016Article
- Expression of cyclin D1 and cyclin E in urothelial bladder carcinoma detected in tissue chips using a quantum dot immunofluorescence technique.Oncology letters · 2015Article
- Upregulated WDR5 promotes proliferation, self-renewal and chemoresistance in bladder cancer via mediating H3K4 trimethylation.Scientific reports · 2015Article
- Methylation-silencing RCC1 expression is associated with tumorigenesis and depth of invasion in gastric cancer.International journal of clinical and experimental pathology · 2015Article
- Associations of NR5A2 gene polymorphisms with the clinicopathological characteristics and survival of gastric cancer.International journal of molecular sciences · 2014Article
- Correlation between ERK1 and STAT3 expression and chemoresistance in patients with conventional osteosarcoma.BMC cancer · 2014Article
- Identification of prefoldin amplification (1q23.3-q24.1) in bladder cancer using comparative genomic hybridization (CGH) arrays of urinary DNA.Journal of translational medicine · 2013Article
- Study on the clinical significance of Argonaute2 expression in colonic carcinoma by tissue microarray.International journal of clinical and experimental pathology · 2013Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
25 authors at 10 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies by comparative genomic hybridization revealed that the 19q13 chromosomal region is frequently amplified in bladder cancer. The cyclin E gene (CCNE), coding for a regulatory subunit of cyclin-dependent kinase 2, has been mapped to 19q13. To investigate the role of cyclin E alterations in bladder cancer, a tissue microarray of 2,317 specimens from 1,842 bladder cancer patients was constructed and analyzed for CCNE amplification by fluorescence in situ hybridization and for cyclin-E protein overexpression by immunohistochemistry. Fluorescence in situ hybridization analysis showed amplification in only 30 of the 1,561 evaluable tumors (1.9%). Amplification was significantly associated with stage and grade (P: < 0.0005 each). Immunohistochemically detectable cyclin E expression was strong in 233 (12.4%), weak in 354 (18.9%), and negative in 1, 286 of the 1,873 interpretable tumors. The majority (62.1%) of CCNE-amplified tumors were strongly immunohistochemistry-positive (P: < 0.0001). The frequency of protein expression increased from stage pTa (22.2%) to pT1 (45.5%; P: < 0.0001) but then decreased for stage pT2-4 (29.4%; P: < 0.0001 for pT1 versus pT2-4). Low cyclin E expression was associated with poor overall survival in all patients (P: < 0.0001), but had no prognostic impact independent of stage. It is concluded that cyclin E overexpression is characteristic to a subset of bladder carcinomas, especially at the stage of early invasion. This analysis of the prognostic impact of CCNE gene amplification and protein expression in >1,500 arrayed bladder cancers was accomplished in a period of 2 weeks, illustrating how the tissue microarray technology remarkably facilitates the evaluation of the clinical relevance of molecular alterations in cancer.
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