ArticleGenetics2000
Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Review
- Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC.Nature microbiology · 2023Article
- Ddp1 Cooperates with Ppx1 to Counter a Stress Response Initiated by Nonvacuolar Polyphosphate.mBio · 2022Article
- Eukaryotic Elongation Factor 3 ProtectsGenes · 2020Article
- Proteomics Answers Which Yeast Genes Are Specific for Baking, Brewing, and Ethanol Production.Bioengineering (Basel, Switzerland) · 2020Article
- Filamentation Regulatory Pathways Control Adhesion-Dependent Surface Responses in Yeast.Genetics · 2019Article
- Discoveries of the phosphatidate phosphatase genes in yeast published in theThe Journal of biological chemistry · 2019Review
- Article
- Two plant-derived aporphinoid alkaloids exert their antifungal activity by disrupting mitochondrial iron-sulfur cluster biosynthesis.The Journal of biological chemistry · 2017Article
- Regulation of cation balance in Saccharomyces cerevisiae.Genetics · 2013Review
- Transcriptional regulation of the Zrg17 zinc transporter of the yeast secretory pathway.The Biochemical journal · 2011Article
- Article
- Repression of sulfate assimilation is an adaptive response of yeast to the oxidative stress of zinc deficiency.The Journal of biological chemistry · 2009Article
- Homeostatic and adaptive responses to zinc deficiency in Saccharomyces cerevisiae.The Journal of biological chemistry · 2009Review
- Analysis of manganese-regulated gene expression in the ligninolytic basidiomycete Ceriporiopsis subvermispora.Current genetics · 2008Article
- Regulation of phospholipid synthesis in Saccharomyces cerevisiae by zinc depletion.Biochimica et biophysica acta · 2007Review
- The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair.Molecular genetics and genomics : MGG · 2007Article
- Regulation of the Saccharomyces cerevisiae EKI1-encoded ethanolamine kinase by zinc depletion.The Journal of biological chemistry · 2006Article
- A role for the Saccharomyces cerevisiae regulation of Ace2 and polarized morphogenesis signaling network in cell integrity.Genetics · 2005Article
- Metal-responsive transcription factors that regulate iron, zinc, and copper homeostasis in eukaryotic cells.Eukaryotic cell · 2004Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biochemistry of human nutritional zinc deficiency remains poorly defined. To characterize in genetic terms how cells respond to zinc deprivation, zinc-regulated genes (ZRG's) were identified in yeast. Gene expression was probed using random lacZ reporter gene fusions, integrated by transposon tagging into a diploid genome as previously described. About half of the genome was examined. Cells exhibiting differences in lacZ expression on low or moderate ( approximately 0. 1 vs. 10 microm) zinc media were isolated and the gene fusions were sequenced. Ribonuclease protection assays demonstrated four- to eightfold increases for the RNAs of the ZAP1, ZRG17 (YNR039c), DPP1, ADH4, MCD4, and YEF3B genes in zinc-deficient cells. All but YEF3B were shown through reporter gene assays to be controlled by a master regulator of zinc homeostasis now known to be encoded by ZAP1. ZAP1 mutants lacked the flocculence and distended vacuoles characteristic of zinc-deficient cells, suggesting that flocculation and vacuolation serve homeostatic functions in zinc-deficient cells. ZRG17 mutants required extra zinc supplementation to repress these phenotypes, suggesting that ZRG17 functions in zinc uptake. These findings illustrate the utility of transposon tagging as an approach for studying regulated gene expression in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.