ArticleMolecular and cellular biochemistry2000
Muscle palmitate uptake and binding are saturable and inhibited by antibodies to FABP(PM).
Article in Molecular and cellular biochemistry, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 74 citations in OpenAlex.
- Regulation of direct adipose tissue free fatty acid storage during mixed meal ingestion and high free fatty acid concentration conditions.American journal of physiology. Endocrinology and metabolism · 2021Trial
- A Critical Assessment of Fasting to Promote Metabolic Health and Longevity.Endocrine reviews · 2025Review
- Relevance of mitochondrial dysfunction in heart disease associated with insulin resistance conditions.Pflugers Archiv : European journal of physiology · 2022Review
- Acute exercise in mice transiently remodels the hepatic lipidome in an intensity-dependent manner.Lipids in health and disease · 2020Article
- Myocardium Metabolism in Physiological and Pathophysiological States: Implications of Epicardial Adipose Tissue and Potential Therapeutic Targets.International journal of molecular sciences · 2020Review
- Pathways of polyunsaturated fatty acid utilization: implications for brain function in neuropsychiatric health and disease.Brain research · 2015Review
- Alterations in oral [1-(14)C] 18:1n-9 distribution in lean wild-type and genetically obese (ob/ob) mice.PloS one · 2015Article
- Regional differences in blood flow, glucose uptake and fatty acid uptake within quadriceps femoris muscle during dynamic knee-extension exercise.European journal of applied physiology · 2013Article
- Age-related expression profile of the SLC27A1 gene in chicken tissues.Molecular biology reports · 2011Article
- Free fatty acid storage in human visceral and subcutaneous adipose tissue: role of adipocyte proteins.Diabetes · 2011Article
- Fatty acid (FFA) transport in cardiomyocytes revealed by imaging unbound FFA is mediated by an FFA pump modulated by the CD36 protein.The Journal of biological chemistry · 2011Article
- The role of membrane fatty-acid transporters in regulating skeletal muscle substrate use during exercise.Sports medicine (Auckland, N.Z.) · 2008Article
- Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle.The Journal of physiology · 2007Article
- Differential effects of contraction and PPAR agonists on the expression of fatty acid transporters in rat skeletal muscle.The Journal of physiology · 2006Article
- Regulation of fatty acid transport and membrane transporters in health and disease.Molecular and cellular biochemistry · 2002Review
- Coordinately regulated expression of FAT/CD36 and FACS1 in rat skeletal muscle.Molecular and cellular biochemistry · 2001Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Studies show that uptake of long-chain fatty acids (LCFA) across the plasma membranes (PM) may occur partly via a carrier-mediated process and that the plasma membrane fatty acid-binding protein (FABP(PM)) may be a component of this system. To test the hypothesis that FABP(PM) is involved in transsarcolemmal transport of LCFA in muscle, we measured palmitate uptake in giant sarcolemmal vesicles and palmitate binding to PM proteins in rat muscles, (1) in the presence of increasing amounts of unbound palmitate and (2) in the absence or presence of antibody to FABP(PM). Both palmitate uptake and binding were found to be saturable functions of the unbound palmitate concentration with calculated Vmax values of 10.5 +/- 1.2 pmol/mg protein/15 sec and 45.6 +/- 2.9 nmol/mg protein/15 min and Km values of 12.8 +/- 3.8 and 18.4 +/- 1.8 nmol/L, respectively. The Vmax values for both palmitate uptake and binding were significantly decreased by 75-79% in the presence of a polyclonal antibody to the rat hepatic FABP(PM). Antibody inhibition was found to be dose-dependent and specific to LCFA. Glucose uptake was not affected by the presence of the antibody to FABP(PM). Palmitate uptake and binding were also inhibited in the presence of trypsin and phloretin. These results support the hypothesis that transsarcolemmal LCFA transport occurs in part by a carrier-mediated process and that FABP(PM) is a component of this process in muscle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.