ArticleProtein science : a publication of the Protein Society2000
Static light scattering studies of OmpF porin: implications for integral membrane protein crystallization.
Article in Protein science : a publication of the Protein Society, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Quantitative Perspectives of Biomolecular Crystallization Thermodynamics.Advances in biochemical engineering/biotechnology · 2026Review
- Quantifying the Influence of Poly(Ethylene glycol) on the Micelle Formation of Nonionic Detergents.ChemPlusChem · 2025Article
- Considering the immunogenicity of PEG: strategies for overcoming issues with PEGylated nanomedicines.Nanomedicine (London, England) · 2025Review
- Unraveling ShuA detergent-induced colloidal behavior in solution: A comprehensive SEC-MALS, SAXS, and SANS study.Protein science : a publication of the Protein Society · 2025Article
- Towards understanding the crystallization of photosystem II: influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides.Photosynthesis research · 2024Article
- Structural Characterization and Modeling of a Respiratory Syncytial Virus Fusion Glycoprotein Nanoparticle Vaccine in Solution.Molecular pharmaceutics · 2021Article
- Applications of the second virial coefficient: protein crystallization and solubility. Corrigendum.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Membrane proteins, detergents and crystals: what is the state of the art?Acta crystallographica. Section F, Structural biology communications · 2014Review
- The significance of G protein-coupled receptor crystallography for drug discovery.Pharmacological reviews · 2011Review
- A high-throughput differential filtration assay to screen and select detergents for membrane proteins.Analytical biochemistry · 2010Article
- In vitro dimerization of the bovine papillomavirus E5 protein transmembrane domain.Biochemistry · 2008Article
- Effects of additives on surfactant phase behavior relevant to bacteriorhodopsin crystallization.Protein science : a publication of the Protein Society · 2006Article
- When worlds colloid.Protein science : a publication of the Protein Society · 2006Article
- Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import.Biophysical journal · 2004Article
- Rationalization of membrane protein crystallization with polyethylene glycol using a simple depletion model.Biophysical journal · 2003Article
- Rapid measurement of protein osmotic second virial coefficients by self-interaction chromatography.Biophysical journal · 2002Article
- A comparative study of monoclonal antibodies. 1. Phase behavior and protein-protein interactions.Biotechnology progressArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integral membrane proteins carry out some of the most important functions of living cells, yet relatively few details are known about their structures. This is due, in large part, to the difficulties associated with preparing membrane protein crystals suitable for X-ray diffraction analysis. Mechanistic studies of membrane protein crystallization may provide insights that will aid in determining future membrane protein structures. Accordingly, the solution behavior of the bacterial outer membrane protein OmpF porin was studied by static light scattering under conditions favorable for crystal growth. The second osmotic virial coefficient (B22) was found to be a predictor of the crystallization behavior of porin, as has previously been found for soluble proteins. Both tetragonal and trigonal porin crystals were found to form only within a narrow window of B22 values located at approximately -0.5 to -2 X 10(-4) mol mL g(-2), which is similar to the "crystallization slot" observed for soluble proteins. The B22 behavior of protein-free detergent micelles proved very similar to that of porin-detergent complexes, suggesting that the detergent's contribution dominates the behavior of protein-detergent complexes under crystallizing conditions. This observation implies that, for any given detergent, it may be possible to construct membrane protein crystallization screens of general utility by manipulating the solution properties so as to drive detergent B22 values into the crystallization slot. Such screens would limit the screening effort to the detergent systems most likely to yield crystals, thereby minimizing protein requirements and improving productivity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.