ArticleThe Journal of cell biology2000
A myosin I is involved in membrane recycling from early endosomes.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 79 citations in OpenAlex.
- Basic-hydrophobic sites are localized in conserved positions inside and outside of PH domains and affect localization ofMolecular biology of the cell · 2020Article
- Gamma secretase orthologs are required for lysosomal activity and autophagic degradation inAutophagy · 2019Article
- WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Dictyostelium discoideum RabS and Rab2 colocalize with the Golgi and contractile vacuole system and regulate osmoregulation.Journal of biosciences · 2016Article
- Selective localization of myosin-I proteins in macropinosomes and actin waves.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Structure of the small Dictyostelium discoideum myosin light chain MlcB provides insights into MyoB IQ motif recognition.The Journal of biological chemistry · 2014Article
- Detecting, visualizing and quantitating the generation of reactive oxygen species in an amoeba model system.Journal of visualized experiments : JoVE · 2013Article
- The myosin motor Myo1c is required for VEGFR2 delivery to the cell surface and for angiogenic signaling.American journal of physiology. Heart and circulatory physiology · 2013Article
- The balance in the delivery of ER components and the vacuolar proton pump to the phagosome depends on myosin IK in Dictyostelium.Molecular & cellular proteomics : MCP · 2012Article
- Molecular basis of dynamic relocalization of Dictyostelium myosin IB.The Journal of biological chemistry · 2012Article
- Muskelin regulates actin filament- and microtubule-based GABA(A) receptor transport in neurons.Neuron · 2011Article
- Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.The EMBO journal · 2010Article
- Identification and characterization of an unusual class I myosin involved in vesicle traffic in Trypanosoma brucei.PloS one · 2010Article
- Leveraging the membrane - cytoskeleton interface with myosin-1.Trends in cell biology · 2010Review
- Control of cell membrane tension by myosin-I.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Copine A, a calcium-dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium.BMC cell biology · 2005Article
- CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling.Molecular biology of the cell · 2005Article
- Interleukin-18 primes the oxidative burst of neutrophils in response to formyl-peptides: role of cytochrome b558 translocation and N-formyl peptide receptor endocytosis.Clinical and diagnostic laboratory immunology · 2005Article
- Functional characterization of myosin I tail regions in Candida albicans.Eukaryotic cell · 2004Article
- Fusion between phagosomes, early and late endosomes: a role for actin in fusion between late, but not early endocytic organelles.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Geometry-based mechanisms have been proposed to account for the sorting of membranes and fluid phase in the endocytic pathway, yet little is known about the involvement of the actin-myosin cytoskeleton. Here, we demonstrate that Dictyostelium discoideum myosin IB functions in the recycling of plasma membrane components from endosomes back to the cell surface. Cells lacking MyoB (myoA(-)/B(-), and myoB(-) cells) and wild-type cells treated with the myosin inhibitor butanedione monoxime accumulated a plasma membrane marker and biotinylated surface proteins on intracellular endocytic vacuoles. An assay based on reversible biotinylation of plasma membrane proteins demonstrated that recycling of membrane components is severely impaired in myoA/B null cells. In addition, MyoB was specifically found on magnetically purified early pinosomes. Using a rapid-freezing cryoelectron microscopy method, we observed an increased number of small vesicles tethered to relatively early endocytic vacuoles in myoA(-)/B(-) cells, but not to later endosomes and lysosomes. This accumulation of vesicles suggests that the defects in membrane recycling result from a disordered morphology of the sorting compartment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.