ArticleThe Journal of cell biology2000
Profilin enhances Cdc42-induced nucleation of actin polymerization.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 131 citations in OpenAlex.
- Profilin and Non-Canonical Wnt Signaling: Coordinating Cytoskeletal Dynamics from Development to Disease.Journal of developmental biology · 2025Review
- Neutrophil attachment via Mac-1 (Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- The intermediate filament protein nestin serves as a molecular hub for smooth muscle cytoskeletal signaling.Respiratory research · 2023Article
- Nearly 30 Years of Animal Models to Study Amyotrophic Lateral Sclerosis: A Historical Overview and Future Perspectives.International journal of molecular sciences · 2021Review
- Actin cytoskeleton deregulation confers midostaurin resistance in FLT3-mutant acute myeloid leukemia.Communications biology · 2021Article
- Profilin2 regulates actin rod assembly in neuronal cells.Scientific reports · 2021Article
- Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction.Respiratory research · 2018Article
- Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs.Journal of innate immunity · 2017Article
- Profilin1 regulates invadopodium maturation in human breast cancer cells.European journal of cell biology · 2015Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Autonomous and in trans functions for the two halves of Srv2/CAP in promoting actin turnover.Cytoskeleton (Hoboken, N.J.) · 2014Article
- The association of cortactin with profilin-1 is critical for smooth muscle contraction.The Journal of biological chemistry · 2014Article
- Processive acceleration of actin barbed-end assembly by N-WASP.Molecular biology of the cell · 2014Article
- Surfing the big WAVE: Insights into the role of WAVE3 as a driving force in cancer progression and metastasis.Seminars in cell & developmental biology · 2013Review
- p70 S6 kinase and actin dynamics: A perspective.Spermatogenesis · 2012Article
- Cytoskeletal actin networks in motile cells are critically self-organized systems synchronized by mechanical interactions.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A.The Journal of cell biology · 2010Article
- Physiologic properties and regulation of the actin cytoskeleton in vascular smooth muscle.Journal of cardiovascular pharmacology and therapeutics · 2008Review
- Diaphanous-related formin 2 and profilin I are required for gastrulation cell movements.PloS one · 2008Article
- The WASP-WAVE protein network: connecting the membrane to the cytoskeleton.Nature reviews. Molecular cell biology · 2007Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
We find that profilin contributes in several ways to Cdc42-induced nucleation of actin filaments in high speed supernatant of lysed neutrophils. Depletion of profilin inhibited Cdc42-induced nucleation; re-addition of profilin restored much of the activity. Mutant profilins with a decreased affinity for either actin or poly-l-proline were less effective at restoring activity. Whereas Cdc42 must activate Wiskott-Aldrich Syndrome protein (WASP) to stimulate nucleation by the Arp2/3 complex, VCA (verpolin homology, cofilin, and acidic domain contained in the COOH-terminal fragment of N-WASP) constitutively activates the Arp2/3 complex. Nucleation by VCA was not inhibited by profilin depletion. With purified N-WASP and Arp2/3 complex, Cdc42-induced nucleation did not require profilin but was enhanced by profilin, wild-type profilin being more effective than mutant profilin with reduced affinity for poly-l-proline. Nucleation by the Arp2/3 complex is a function of the free G-actin concentration. Thus, when profilin addition decreased the free G-actin concentration, it inhibited Cdc42- and VCA-induced nucleation. However, when profilin was added with G-actin in a ratio that maintained the initial free G-actin concentration, it increased the rate of both Cdc42- and VCA-induced nucleation. This enhancement, also seen with purified proteins, was greatest when the free G-actin concentration was low. These data suggest that under conditions present in intact cells, profilin enhances nucleation by activated Arp2/3 complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.