ArticleThe Journal of cell biology2000
Nuclei and microtubule asters stimulate maturation/M phase promoting factor (MPF) activation in Xenopus eggs and egg cytoplasmic extracts.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Centrosome - a promising anti-cancer target.Biologics : targets & therapy · 2016Review
- The centrosome and its duplication cycle.Cold Spring Harbor perspectives in biology · 2015Review
- Calcium signaling and meiotic exit at fertilization in Xenopus egg.International journal of molecular sciences · 2014Review
- Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast.Molecular biology of the cell · 2014Article
- Causes and consequences of centrosome abnormalities in cancer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Organization of early frog embryos by chemical waves emanating from centrosomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Optimisation of an oviposition protocol employing human chorionic and pregnant mare serum gonadotropins in the barred frog Mixophyes fasciolatus (Myobatrachidae).Reproductive biology and endocrinology : RB&E · 2012Article
- Activation of cyclin B1-Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis.The Journal of cell biology · 2010Article
- Centrosome and retroviruses: the dangerous liaisons.Retrovirology · 2007Review
- Fertilization and preimplantation development of mouse oocytes after prolonged incubation with caffeine.Reproductive medicine and biology · 2004Article
- Localization and dynamics of Cdc2-cyclin B during meiotic reinitiation in starfish oocytes.Molecular biology of the cell · 2003Article
- Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe.Genes & development · 2003Article
- Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes.The Journal of cell biology · 2003Article
- Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts.Molecular biology of the cell · 2003Article
- Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis.Molecular biology of the cell · 2002Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although maturation/M phase promoting factor (MPF) can activate autonomously in Xenopus egg cytoplasm, indirect evidence suggests that nuclei and centrosomes may focus activation within the cell. We have dissected the contribution of these structures to MPF activation in fertilized eggs and in egg fragments containing different combinations of nuclei, centrosomes, and microtubules by following the behavior of Cdc2 (the kinase component of MPF), the regulatory subunit cyclin B, and the activating phosphatase Cdc25. The absence of the entire nucleus-centrosome complex resulted in a marked delay in MPF activation, whereas the absence of the centrosome alone caused a lesser delay. Nocodazole treatment to depolymerize microtubules through first interphase had an effect equivalent to removing the centrosome. Furthermore, microinjection of isolated centrosomes into anucleate eggs promoted MPF activation and advanced the onset of surface contraction waves, which are close indicators of MPF activation and could be triggered by ectopic MPF injection. Finally, we were able to demonstrate stimulation of MPF activation by the nucleus-centriole complex in vitro, as low concentrations of isolated sperm nuclei advanced MPF activation in cycling cytoplasmic extracts. Together these results indicate that nuclei and microtubule asters can independently stimulate MPF activation and that they cooperate to enhance activation locally.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.