ArticleProceedings of the National Academy of Sciences of the United States of America2000
Replication-dependent early meiotic requirement for Spo11 and Rad50.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- Adaptive Spo11 RNA editing gate optimizes meiosis I pace and mitotic proliferation while preserving ascospore formation.Science advances · 2026Article
- Developmental stage dominates cell-type identity and reveals a chromatin regulatory function for Rad50 in Drosophila.Nucleic acids research · 2026Article
- Insight into meiotic DNA end resection: Mechanisms and regulation.DNA repair · 2025Review
- Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana.PLoS genetics · 2022Article
- Chromosome Organization in Early Meiotic Prophase.Frontiers in cell and developmental biology · 2021Review
- Recruitment of Rec8, Pds5 and Rad61/Wapl to meiotic homolog pairing, recombination, axis formation and S-phase.Nucleic acids research · 2019Article
- FgRad50 Regulates Fungal Development, Pathogenicity, Cell Wall Integrity and the DNA Damage Response inFrontiers in microbiology · 2019Article
- Transient reduction of DNA methylation at the onset of meiosis in male mice.Epigenetics & chromatin · 2018Article
- Comparative transcriptomics of the model mushroom Coprinopsis cinerea reveals tissue-specific armories and a conserved circuitry for sexual development.BMC genomics · 2014Article
- An MSH4 homolog, stpp1, from Pleurotus pulmonarius is a "silver bullet" for resolving problems caused by spores in cultivated mushrooms.Applied and environmental microbiology · 2013Article
- Competing roles of DNA end resection and non-homologous end joining functions in the repair of replication-born double-strand breaks by sister-chromatid recombination.Nucleic acids research · 2013Article
- Meiotic recombination protein Rec12: functional conservation, crossover homeostasis and early crossover/non-crossover decision.Nucleic acids research · 2011Article
- Analysis of the Basidiomycete Coprinopsis cinerea reveals conservation of the core meiotic expression program over half a billion years of evolution.PLoS genetics · 2010Article
- Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice.Genes & development · 2010Article
- A high throughput genetic screen identifies new early meiotic recombination functions in Arabidopsis thaliana.PLoS genetics · 2009Article
- The axial element protein HTP-3 promotes cohesin loading and meiotic axis assembly in C. elegans to implement the meiotic program of chromosome segregation.Genes & development · 2009Article
- Meiotic localization of Mre11 and Rad50 in wild type, spo11-1, and MRN complex mutants of Coprinus cinereus.Chromosoma · 2009Article
- Article
- Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis.Genome dynamics and stability · 2008Article
- An expanded inventory of conserved meiotic genes provides evidence for sex in Trichomonas vaginalis.PloS one · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Spo11 and the Rad50-Mre11 complex have been indirectly implicated in processes associated with DNA replication. These proteins also have been shown to have early meiotic roles essential for the formation of a programmed DNA double-strand break known in Saccharomyces cerevisiae to initiate meiotic recombination. In both S. cerevisiae and the basidiomycete Coprinus cinereus, spo11 and rad50 mutants are defective in chromosome synapsis during meiosis. Here we demonstrate that a partial restoration of synapsis occurs in C. cinereus spo11 and rad50 mutants if premeiotic DNA replication is prevented. Double mutants were constructed with spo11-1 or rad50-4 and another mutant, spo22-1, which does not undergo premeiotic DNA replication. In both cases, we observed an increase in the percentage of nuclei containing synaptonemal complex (SC) structures, with concomitant decreases in the percentage of nuclei containing axial elements (AE) only or no structures. Both types of double mutants demonstrated significant increases in the average numbers of AE and SC, although SC-containing nuclei did not on average contain more AE than did nuclei showing no synapsis. Our results show that Spo11-induced recombination is not absolutely required for synapsis in C. cinereus, and that the early meiotic role of both Spo11 and Rad50 in SC formation partially depends on premeiotic S phase. This dependency likely reflects either a requirement for these proteins imposed by the premeiotic replication process itself or a requirement for these proteins in synapsis when a sister chromatid (the outcome of DNA replication) is present.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.