ArticleClinical and diagnostic laboratory immunology2000
Serological evaluation of thin-layer immunoassay-enzyme-linked immunosorbent assay for antibody detection in human trichinellosis.
Article in Clinical and diagnostic laboratory immunology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Racemose Neurocysticercosis Presenting As Vasculitic Infarct and Obstructive Hydrocephalus: A Rare Presentation.Cureus · 2025Article
- Effective Laboratory Diagnosis of Parasitic Infections of the Gastrointestinal Tract: Where, When, How, and What Should We Look For?Diagnostics (Basel, Switzerland) · 2024Review
- Thin Layer Immunoassay: An Economical Approach to DiagnoseDiagnostics (Basel, Switzerland) · 2023Article
- Serological testing forFood and waterborne parasitology · 2022Article
- Serological tools for detection ofOne health (Amsterdam, Netherlands) · 2016Review
- Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis.Clinical and vaccine immunology : CVI · 2008Article
- Application and assessment of a dipstick assay in the diagnosis of hydatidosis and trichinosis.Parasitology research · 2004Article
- Development and evaluation of a Western blot kit for diagnosis of human trichinellosis.Clinical and diagnostic laboratory immunology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new immunoenzymatic test, named the thin-layer immunoassay-enzyme-linked immunosorbent assay (TIA-ELISA), was evaluated for antibody detection in human trichinellosis using excretion and secretion products prepared from Trichinella spiralis muscle larvae. Serum samples from people with positive muscle biopsies or symptoms compatible with the disease (n = 8 or 26, respectively), all reactive in enzyme-linked immunoelectrotransfer blot assay (EITB), as well as 67 serum samples from healthy, EITB-negative people, were tested in an ELISA and TIA-ELISA. TIA-ELISA was performed in polystyrene plastic petri dishes by adding dots of 10 microl each of antigen (7 microg/ml) followed by adding diluted serum and the conjugate. Finally, the substrate mixed with agar was added to develop the reaction. Enzymatic by-products were easily detected by the naked eye as defined dots. Sensitivity and specificity were 76 and 94% for ELISA, and both parameters were 91% for TIA-ELISA. The kappa correlation indices for both tests in relation to EITB were 0.73 and 0.80, respectively. The TIA-ELISA can be carried out with common laboratory equipment in 3 h and uses lower quantities of antigen than EITB and ELISA. Since TIA-ELISA is easy to perform, cheap, sensitive, and specific, the test could be an acceptable alternative to use in clinical laboratories lacking specialized equipment needed for ELISA and EITB and in field studies for antibody detection in human trichinellosis.
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