ArticleNature biotechnology2000
Cytokine production in motor neurons by poliovirus replicon vector gene delivery.
Article in Nature biotechnology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Viral Replicon Systems and Their Biosafety Aspects.Applied biosafety : journal of the American Biological Safety Association · 2023Review
- Viral vectors for therapy of neurologic diseases.Neuropharmacology · 2017Review
- CD34+ hematopoietic stem cells support entry and replication of poliovirus: a potential new gene introduction route.Cancer gene therapy · 2013Article
- Polio, still lurking in the shadows.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- A Sabin 1 poliovirus-based vaccine vector transfects Vero cells with high efficiency.Cytotechnology · 2007Article
- Long-term gene expression in dividing and nondividing cells using SV40-derived vectors.Molecular biotechnology · 2006Review
- Glial activation and segmental upregulation of interleukin-1beta (IL-1beta) in the rat spinal cord after surgical incision.Neurochemical research · 2006Article
- Comparative analysis of genomic HSV vectors for gene delivery to motor neurons following peripheral inoculation in vivo.Gene therapy · 2004Article
- Oncolytic viruses: clinical applications as vectors for the treatment of malignant gliomas.Journal of neuro-oncology · 2003Review
- Expression of brain-derived neurotrophic factor in the central nervous system of mice using a poliovirus-based vector.Journal of neurovirology · 2002Article
- Novel design architecture for genetic stability of recombinant poliovirus: the manipulation of G/C contents and their distribution patterns increases the genetic stability of inserts in a poliovirus-based RPS-Vax vector system.Journal of virology · 2002Article
- Protection against simian immunodeficiency virus vaginal challenge by using Sabin poliovirus vectors.Journal of virology · 2001Article
- Spinal muscular atrophy: present state.Brain pathology (Zurich, Switzerland) · 2001Review
- Future directions in alcoholism research. Genomics and gene transfer.Alcohol research & health : the journal of the National Institute on Alcohol Abuse and Alcoholism · 2000Review
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4 authors at 1 institution in 1 country.
Funding
Abstract
Poliovirus replicon vectors transiently express foreign proteins selectively in motor neurons of the anterior horn of the spinal cord. Here we intraspinally inoculated mice transgenic for the poliovirus receptor (PVR) with replicons encoding murine tumor necrosis factor alpha (mTNF-alpha). We detected high-level expression of mTNF-alpha in the spinal cords of these animals at 8-12 h post inoculation; this returned to background by 72 h. The mice exhibited ataxia and tail atony, whereas animals given a replicon encoding green fluorescent protein (GFP) exhibited no neurological symptoms. Histology of spinal cords from mice given the replicon encoding mTNF-alpha revealed neuronal chromatolysis, reactive astrogliosis, decreased expression of myelin basic protein, and demyelination. These animals recovered with only slight residual damage. This study shows that replicon vectors have potential for targeted delivery of therapeutic proteins to the central nervous system and provide a new approach for treatment of spinal cord trauma and neurological disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.