ArticleThe EMBO journal2000
Ca(2+) signalling is not required for chemotaxis in Dictyostelium.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
58 citing papers in PubMed, 118 citations in OpenAlex.
- Establishing functional giant Dictyostelium cells reveals front-rear polarity in intracellular signaling.Communications biology · 2026Article
- A close unicellular animal relative and predator of schistosomes exhibits chemokinesis in response to proteins and peptides from its prey.PLoS pathogens · 2025Article
- Two endogenousProceedings of the National Academy of Sciences of the United States of America · 2025Article
- mTORC1 pathway activity biases cell fate choice.Scientific reports · 2024Article
- Ion Signaling in Cell Motility and Development inBiomolecules · 2024Review
- Polycystin-2 Mediated Calcium Signalling in theCells · 2024Article
- Quantitative Monitoring of GPCR-Mediated Spatiotemporal IPMethods in molecular biology (Clifton, N.J.) · 2024Article
- Calcium responses to external mechanical stimuli in the multicellular stage of Dictyostelium discoideum.Scientific reports · 2022Article
- TheFrontiers in cell and developmental biology · 2022Article
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- Membrane Targeting of C2GAP1 EnablesFrontiers in cell and developmental biology · 2021Article
- Review
- Pressure sensing through Piezo channels controls whether cells migrate with blebs or pseudopods.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
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- The acidocalcisome inositol-1,4,5-trisphosphate receptor ofThe Journal of biological chemistry · 2019Article
- An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.Molecular biology of the cell · 2019Article
- A study of wound repair in Dictyostelium cells by using novel laserporation.Scientific reports · 2018Article
- A polycystin-type transient receptor potential (Trp) channel that is activated by ATP.Biology open · 2017Article
- Secreted Cyclic Di-GMP Induces Stalk Cell Differentiation in the Eukaryote Dictyostelium discoideum.Journal of bacteriology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dictyostelium cells can move rapidly towards a source of cyclic-AMP (cAMP). This chemoattractant is detected by G-protein-linked receptors, which trigger a signalling cascade including a rapid influx of Ca(2+). We have disrupted an inositol 1,4,5-trisphosphate (InsP(3)) receptor-like gene, iplA, to produce null cells in which Ca(2+) entry in response to chemoattractants is abolished, as is the normal increase in free cytosolic Ca(2+) ([Ca(2+)](c)) that follows chemotactic stimulation. However, the resting [Ca(2+)](c) is similar to wild type. This mutant provides a test for the role of Ca(2+) influx in both chemotaxis and the signalling cascade that controls it. The production of cyclic-GMP and cAMP, and the activation of the MAP kinase, DdERK2, triggered from the cAMP receptor, are little perturbed in the mutant; mobilization of actin into the cytoskeleton also follows similar kinetics to wild type. Mutant cells chemotax efficiently towards cAMP or folic acid and their sensitivity to cAMP is similar to wild type. Finally, they move at similar speeds to wild-type cells, with or without chemoattractant. We conclude that Ca(2+) signalling is not necessary for chemotaxis to cAMP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.