ArticleThe EMBO journal2000
Nonsense-mediated decay of glutathione peroxidase 1 mRNA in the cytoplasm depends on intron position.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 89 citations in OpenAlex.
- PRMT1-SFPQ regulates intron retention to control matrix gene expression during craniofacial development.eLife · 2026Article
- Nonsense-mediated mRNA decay: a key regulatory system engaged in cancer.Cell communication and signaling : CCS · 2025Review
- Development of a sensor to detect methylmercury toxicity.Scientific reports · 2024Article
- FMRP-mediated spatial regulation of physiologic NMD targets in neuronal cells.Genome biology · 2024Review
- From Yeast to Mammals, the Nonsense-Mediated mRNA Decay as a Master Regulator of Long Non-Coding RNAs Functional Trajectory.Non-coding RNA · 2021Review
- Rescue effects of Se-enriched rice on physiological and biochemical characteristics in cadmium poisoning mice.Environmental science and pollution research international · 2021Article
- Neonatal Selenoenzyme Expression Is Variably Susceptible to Duration of Maternal Selenium Deficiency.Antioxidants (Basel, Switzerland) · 2021Article
- Methylmercury-Mediated Oxidative Stress and Activation of the Cellular Protective System.Antioxidants (Basel, Switzerland) · 2020Review
- Review
- The utilization of selenocysteine-tRNATranslation (Austin, Tex.) · 2017Article
- Review
- Nonsense-mediated decay factors are involved in the regulation of selenoprotein mRNA levels during selenium deficiency.RNA (New York, N.Y.) · 2014Article
- The molecular biology of selenocysteine.Biomolecular concepts · 2013Review
- Knockout of SOD1 promotes conversion of selenocysteine to dehydroalanine in murine hepatic GPX1 protein.Free radical biology & medicine · 2011Article
- Regulation of H-ras splice variant expression by cross talk between the p53 and nonsense-mediated mRNA decay pathways.Molecular and cellular biology · 2007Article
- Aminoglycosides decrease glutathione peroxidase-1 activity by interfering with selenocysteine incorporation.The Journal of biological chemistry · 2006Article
- A 3' UTR sequence stabilizes termination codons in the unspliced RNA of Rous sarcoma virus.RNA (New York, N.Y.) · 2006Article
- Efficient downregulation of immunoglobulin mu mRNA with premature translation-termination codons requires the 5'-half of the VDJ exon.Nucleic acids research · 2004Article
- Review
- Nonsense-containing mRNAs that accumulate in the absence of a functional nonsense-mediated mRNA decay pathway are destabilized rapidly upon its restitution.Molecular and cellular biology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
mRNA for glutathione peroxidase 1 (GPx1) is subject to cytoplasmic nonsense-mediated decay (NMD) when the UGA selenocysteine (Sec) codon is recognized as nonsense. Here, we demonstrate by moving the sole intron of the GPx1 gene that either the Sec codon or a TAA codon in its place elicits NMD when located >/=59 bp but not </=43 bp upstream of the intron. Therefore, the exon-exon junction of GPx1 mRNA positions the boundary between nonsense codons that do and do not elicit NMD, as has been shown for the 3'-most junctions of mRNAs subject to nucleus-associated NMD. We also demonstrate by using a regulatable promoter to drive GPx1 gene expression that cytoplasmic NMD is characteristic of steady-state mRNA, in contrast to nucleus-associated NMD. These findings clarify the mechanistic relationship between cytoplasmic and nucleus-associated NMD and offer the first demonstration that nuclear introns can influence cytoplasmic NMD. Finally, by analyzing hybrid GPx1 genes, we disprove the idea that the cellular site of NMD is determined by the efficiency of translation initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.