ArticleThe EMBO journal2000
Src-mediated activation of alpha-diacylglycerol kinase is required for hepatocyte growth factor-induced cell motility.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 89 citations in OpenAlex.
- Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3.Thoracic cancer · 2023Article
- Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction.Frontiers in immunology · 2023Article
- Review
- Elusive structure of mammalian DGKs.Advances in biological regulation · 2022Review
- UsingFrontiers in cell and developmental biology · 2021Review
- Molecular Pathways: Targeting Diacylglycerol Kinase Alpha in Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Article
- Physiological Signaling and Structure of the HGF Receptor MET.Biomedicines · 2014Review
- Diacylglycerol kinase α promotes 3D cancer cell growth and limits drug sensitivity through functional interaction with Src.Oncotarget · 2014Article
- Isoform 5 of PIPKIγ regulates the endosomal trafficking and degradation of E-cadherin.Journal of cell science · 2014Article
- Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells.Biochimica et biophysica acta · 2014Article
- EF-hand motifs of diacylglycerol kinase α interact intra-molecularly with its C1 domains.FEBS open bio · 2014Article
- The diacylglycerol kinase α/atypical PKC/β1 integrin pathway in SDF-1α mammary carcinoma invasiveness.PloS one · 2014Article
- A miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.Neuro-oncology · 2013Article
- cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1.Journal of lipid research · 2013Article
- Diacylglycerol kinase α controls RCP-dependent integrin trafficking to promote invasive migration.The Journal of cell biology · 2012Article
- SAP-mediated inhibition of diacylglycerol kinase α regulates TCR-induced diacylglycerol signaling.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- A dual role for diacylglycerol kinase generated phosphatidic acid in autoantibody-induced neutrophil exocytosis.Molecular medicine (Cambridge, Mass.) · 2011Article
- Diacylglycerol kinase alpha mediates HGF-induced Rac activation and membrane ruffling by regulating atypical PKC and RhoGDI.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Enzymatic measurement of phosphatidic acid in cultured cells.Journal of lipid research · 2009Article
- Mammalian diacylglycerol kinases: molecular interactions and biological functions of selected isoforms.Biochimica et biophysica acta · 2009Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diacylglycerol kinases are involved in cell signaling, either as regulators of diacylglycerol levels or as intracellular signal-generating enzymes. However, neither their role in signal transduction nor their biochemical regulation has been elucidated. Hepatocyte growth factor (HGF), upon binding to its tyrosine kinase receptor, activates multiple signaling pathways stimulating cell motility, scattering, proliferation and branching morphogenesis. Herein we demonstrate that: (i) the enzymatic activity of alpha-diacylglycerol kinase (alphaDgk) is stimulated by HGF in epithelial, endothelial and alphaDgk-transfected COS cells; (ii) cellular expression of an alphaDgk kinase-defective mutant inhibits activation of endogenous alphaDgk acting as dominant negative; (iii) specific inhibition of alphaDgk prevents HGF-induced cell movement of endothelial cells; (iv) HGF induces the association of alphaDgk in a complex with Src, whose tyrosine kinase activity is required for alphaDgk activation by HGF; (v) Src wild type stimulates alphaDgk activity in vitro; and (vi) alphaDgk can be tyrosine phosphorylated in intact cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.