ArticleThe Biochemical journal2000
Diffusion control of protein phosphorylation in signal transduction pathways.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 76 citations in OpenAlex.
- Intracellular transport dynamics revealed by single-particle tracking.Biophysics reports · 2021Article
- Proofreading through spatial gradients.eLife · 2020Article
- Dynamics of Posttranslational Modification Systems: Recent Progress and Future Directions.Biophysical journal · 2018Review
- Article
- Dynamics and stability of a three-dimensional model of cell signal transduction.Journal of mathematical biology · 2013Article
- Spatial aspects in the SMAD signaling pathway.Journal of mathematical biology · 2013Article
- The energy costs of insulators in biochemical networks.Biophysical journal · 2013Article
- Cytoplasmic electric fields and electroosmosis: possible solution for the paradoxes of the intracellular transport of biomolecules.PloS one · 2013Review
- Spatial and temporal sensing limits of microtubule polarization in neuronal growth cones by intracellular gradients and forces.Biophysical journal · 2012Article
- Physical constraints on the establishment of intracellular spatial gradients in bacteria.BMC biophysics · 2012Article
- Formation of morphogen gradients: local accumulation time.Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Design principles of nuclear receptor signaling: how complex networking improves signal transduction.Molecular systems biology · 2010Article
- How long does it take to establish a morphogen gradient?Biophysical journal · 2010Article
- Coulomb interactions between cytoplasmic electric fields and phosphorylated messenger proteins optimize information flow in cells.PloS one · 2010Article
- A Cut Cell Method for Simulating Spatial Models of Biochemical Reaction Networks in Arbitrary Geometries.Communications in applied mathematics and computational science · 2010Article
- Subcellular targeting strategies for drug design and delivery.Nature reviews. Drug discovery · 2010Review
- SIMULATING BIOCHEMICAL SIGNALING NETWORKS IN COMPLEX MOVING GEOMETRIES.SIAM journal on scientific computing : a publication of the Society for Industrial and Applied Mathematics · 2010Article
- Effects of chronic overload on muscle hypertrophy and mTOR signaling in young adult and aged rats.The journals of gerontology. Series A, Biological sciences and medical sciences · 2009Article
- Signaling cascades as cellular devices for spatial computations.Journal of mathematical biology · 2009Article
- Insight or illusion? Seeing inside the cell with mesoscopic simulations.HFSP journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Multiple signalling proteins are phosphorylated and dephosphorylated at separate cellular locations, which potentially causes spatial gradients of phospho-proteins within the cell. We have derived relationships that enable us to estimate the extent to which a protein kinase, a phosphatase and the diffusion of signalling proteins control the protein phosphorylation flux and the phospho-protein gradient. Two different cellular geometries were analysed: (1) the kinase is located on one planar membrane and the phosphatase on a second parallel planar membrane, and (2) the kinase is located on the plasma membrane of a spherical cell and the phosphatase is distributed homogeneously in the cytoplasm. We demonstrate that the control contribution of protein diffusion is potentially significant, given the measured rates for protein kinases, phosphatases and diffusion. If the distance between the membranes is 1 microm or greater, the control by diffusion can reach 33% or more, with the rest of the control (67%) shared by the kinase and the phosphatase. At distances of less than 0.1 microm, diffusion does not limit protein phosphorylation. For a spherical cell of radius 10 microm, a protein diffusion coefficient of 10(-8) cm(2). s(-1) and rate constants for the kinase and the phosphatase of approx. 1 s(-1), control over the phosphorylation flux resides mainly with the phosphatase and protein diffusion, with approximately equal contributions of each of these. The ratio of phospho-protein concentrations at the cell membrane and the cell centre (the dynamic compartmentation of the phospho-protein) is shown to be controlled by the rates of the protein phosphatase and of diffusion. The kinase can contribute significantly to the control of the absolute value of the phospho-protein gradient.
Indexed as
Identifiers
10970807PMC1221325W2057554308What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.