ArticleThe Biochemical journal2000
Cytosine methylation of an Sp1 site contributes to organ-specific and cell-specific regulation of expression of the lung epithelial gene t1alpha.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- DNA Methylation Inhibits the Expression of CFSH in Mud Crab.Frontiers in endocrinology · 2020Article
- DNA methylation status of TBX20 in patients with tetralogy of Fallot.BMC medical genomics · 2019Article
- Cell-specific expression of aquaporin-5 (Aqp5) in alveolar epithelium is directed by GATA6/Sp1 via histone acetylation.Scientific reports · 2017Article
- Twist1/Dnmt3a and miR186 establish a regulatory circuit that controls inflammation-associated prostate cancer progression.Oncogenesis · 2017Article
- DNMT3B modulates the expression of cancer-related genes and downregulates the expression of the gene VAV3 via methylation.American journal of cancer research · 2017Article
- Epigenetic modification of TLR4 promotes activation of NF-κB by regulating methyl-CpG-binding domain protein 2 and Sp1 in gastric cancer.Oncotarget · 2016Article
- Tetralogy of Fallot and Hypoplastic Left Heart Syndrome - Complex Clinical Phenotypes Meet Complex Genetic Networks.Current genomics · 2015Article
- Transcriptional regulation of oncogenic protein kinase Cϵ (PKCϵ) by STAT1 and Sp1 proteins.The Journal of biological chemistry · 2014Article
- Association of promoter methylation statuses of congenital heart defect candidate genes with Tetralogy of Fallot.Journal of translational medicine · 2014Article
- Specificity protein 1 (Sp1)-dependent activation of the synapsin I gene (SYN1) is modulated by RE1-silencing transcription factor (REST) and 5'-cytosine-phosphoguanine (CpG) methylation.The Journal of biological chemistry · 2013Article
- A role for DNA methylation in regulation of EphA5 receptor expression in the mouse retina.Vision research · 2011Article
- Characterization of the human Activin-A receptor type II-like kinase 1 (ACVRL1) promoter and its regulation by Sp1.BMC molecular biology · 2010Article
- Epigenetic mechanisms modulate thyroid transcription factor 1-mediated transcription of the surfactant protein B gene.The Journal of biological chemistry · 2010Article
- Epigenetics, oxidative stress, and Alzheimer disease.Free radical biology & medicine · 2009Review
- Bioinformatic interrogation of expression array data to identify nutritionally regulated genes potentially modulated by DNA methylation.Genes & nutrition · 2008Article
- Sp1/Sp3 and DNA-methylation contribute to basal transcriptional activation of human podoplanin in MG63 versus Saos-2 osteoblastic cells.BMC molecular biology · 2007Article
- Role of transcription factor Sp1 and CpG methylation on the regulation of the human podocalyxin gene promoter.BMC molecular biology · 2006Article
- CpG methylation at the USF-binding site is important for the liver-specific transcription of the chipmunk HP-27 gene.The Biochemical journal · 2006Article
- On the epigenetic regulation of the human reelin promoter.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Several recent observations have suggested that cytosine methylation has a role in the in vivo transcriptional regulation of cell-specific genes in normal cells. We hypothesized that methylation regulates T1alpha, a gene expressed primarily in lung in adult rodents. In fetuses T1alpha is expressed in several organs, including the entire nervous system, but during development its expression is progressively restricted to lung alveolar type I epithelial cells, some osteoblasts and choroid plexus. Here we report that T1alpha is methylated at a key Sp1 site in the proximal promoter in cells and organs, including brain, where no gene expression is detectable. Conversely, in T1alpha-expressing cells, these sites are not methylated. In embryonic brain T1alpha is unmethylated and expressed; in adult brain the gene is methylated and not expressed. In lung epithelial cell lines, methylation of the T1alpha promoter in vitro decreases expression by approx. 50% (the maximum suppression being 100%). Analysis of mutated promoter constructs indicates that a single Sp1 site in the proximal promoter provides all or most of the methylation-sensitive gene silencing. We conclude that, in addition to regulation by transcription factors, cytosine methylation has a role in the complex expression patterns of this gene in intact animals and primary cells.
Indexed as
Identifiers
10970805PMC1221323W2092952537What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.