ArticleThe Biochemical journal2000
Identification of transient glycosylation alterations of sialylated mucin oligosaccharides during infection by the rat intestinal parasite Nippostrongylus brasiliensis.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- The story of the SdGlycoconjugate journal · 2023Review
- Gram-Negative Bacterial Endotoxin LPS Induces NeuGc Loss through Ets1-Dependent Downregulation of Intestine-SpecificInternational journal of molecular sciences · 2020Article
- Profiling and Structural Characterization of High Neu5Gc or Sulfate-containing O-glycans from Hyla Rabbit Intestinal Mucin.Molecules (Basel, Switzerland) · 2019Article
- Intestine specific regulation of pig cytidine-5'-monophospho-N-acetylneuraminic acid hydroxylase gene for N-glycolylneuraminic acid biosynthesis.Scientific reports · 2019Article
- A sticky end for gastrointestinal helminths; the role of the mucus barrier.Parasite immunology · 2018Review
- Intestinal Muc2 mucin O-glycosylation is affected by microbiota and regulated by differential expression of glycosyltranferases.Glycobiology · 2017Article
- Immune-driven alterations in mucin sulphation is an important mediator of Trichuris muris helminth expulsion.PLoS pathogens · 2017Article
- Housekeeping promoter 5'pcmah-2 of pig CMP-N-acetylneuraminic acid hydroxylase gene for NeuGc expression.Glycoconjugate journal · 2016Article
- Goblet cells and mucins: role in innate defense in enteric infections.Pathogens (Basel, Switzerland) · 2013Review
- Mucin-Related Molecular Responses of Bronchial Epithelial Cells in Rats Infected with the Nematode Nippostrongylus brasiliensis.ISRN parasitology · 2013Article
- Structural analysis of N- and O-glycans released from glycoproteins.Nature protocols · 2012Article
- Helminthic therapy: improving mucosal barrier function.Trends in parasitology · 2012Review
- The monoclonal antibody HCM31 specifically recognises the Sd(a) tetrasaccharide in goblet cell mucin.FEBS open bio · 2012Article
- A perspective on the emergence of sialic acids as potent determinants affecting leishmania biology.Molecular biology international · 2011Article
- Selection on cis-regulatory variation at B4galnt2 and its influence on von Willebrand factor in house mice.Molecular biology and evolution · 2009Article
- Alteration of the expression profiles of acidic mucin, sialytransferase, and sulfotransferases in the intestinal epithelium of rats infected with the nematode Nippostrongylus brasiliensis.Parasitology research · 2008Article
- Mucins in the mucosal barrier to infection.Mucosal immunology · 2008Review
- Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies.Current opinion in immunology · 2007Review
- Expression profiling reveals novel innate and inflammatory responses in the jejunal epithelial compartment during infection with Trichinella spiralis.Infection and immunity · 2004Article
- Regulation of N-glycolylneuraminic acid biosynthesis in developing pig small intestine.The Biochemical journal · 2003Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sialylation of the oligosaccharides from small-intestinal mucins during a 13-day infectious cycle was studied in Sprague-Dawley rats with the parasite Nippostrongylus brasiliensis. Sialic acid analysis and release, permethylation and analysis by GC-MS of the sialylated oligosaccharides isolated from the 'insoluble' mucin complex revealed a relative decrease (4-7-fold) of N-glycolylneuraminic acid compared with N-acetylneuraminic acid just before parasite expulsion. Northern blots showed that this effect was due to the decreased expression of a hydroxylase converting CMP-N-acetylneuraminic acid into CMP-N-glycolylneuraminic acid. Analysis of other rat strains showed that this parasite infection also caused the same effect in these animals. Detailed analysis of infected Sprague-Dawley rats revealed four sialylated oligosaccharides not found in the uninfected animals. These new oligosaccharides were characterized in detail and all shown to contain the trisaccharide epitope NeuAc/NeuGcalpha2-3(GalNAcbeta1-4)Galbeta1 (where NeuGc is N-glycolyl neuraminic acid). This epitope is similar to the Sd(a)- and Cad-type blood-group antigens and suggests that the infection causes the induction of a GalNAcbeta1-4 glycosyltransferase. This model for an intestinal infection suggests that the glycosylation of intestinal mucins is a dynamic process being modulated by the expression of specific enzymes during an infection process.
Indexed as
Identifiers
10970796PMC1221314W2084457519What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.