ArticleThe Journal of physiology2000
Molecular determinants of Ca2+-dependent K+ channel function in rat dorsal vagal neurones.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 88 citations in OpenAlex.
- Small-Conductance CaNeuroscience bulletin · 2026Review
- Overexpression of small-conductance Ca2+-activated K+ channel 2 attenuates pain-like behavior in female mice with cystitis.JCI insight · 2026Article
- Brainstem activation of GABAFrontiers in neuroscience · 2022Article
- Small-Conductance CaFrontiers in pharmacology · 2020Article
- Ethanol Withdrawal Drives Anxiety-Related Behaviors by Reducing M-type Potassium Channel Activity in the Lateral Habenula.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2017Article
- Polysialic Acid Regulates Sympathetic Outflow by Facilitating Information Transfer within the Nucleus of the Solitary Tract.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Complementary functions of SK and Kv7/M potassium channels in excitability control and synaptic integration in rat hippocampal dentate granule cells.The Journal of physiology · 2014Article
- Characteristics of single large-conductance Ca2+-activated K+ channels and their regulation of action potentials and excitability in parasympathetic cardiac motoneurons in the nucleus ambiguus.American journal of physiology. Cell physiology · 2014Article
- Melanocortin signaling in the brainstem influences vagal outflow to the stomach.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The relationship between functional inhibition and binding for K(Ca)2 channel blockers.PloS one · 2013Article
- Properties of urethral rhabdosphincter motoneurons and their regulation by noradrenaline.The Journal of physiology · 2010Article
- Small conductance Ca2+-activated K+ channels regulate firing properties and excitability in parasympathetic cardiac motoneurons in the nucleus ambiguus.American journal of physiology. Cell physiology · 2010Article
- Cloning and distribution of Ca2+-activated K+ channels in lobster Panulirus interruptus.Neuroscience · 2010Article
- Maternal diabetes increases small conductance Ca2+-activated K+ (SK) currents that alter action potential properties and excitability of cardiac motoneurons in the nucleus ambiguus.Journal of neurophysiology · 2010Article
- BKCa currents are enriched in a subpopulation of adult rat cutaneous nociceptive dorsal root ganglion neurons.The European journal of neuroscience · 2010Article
- Mutation in the myelin proteolipid protein gene alters BK and SK channel function in the caudal medulla.Respiratory physiology & neurobiology · 2009Article
- Excitability of paraventricular nucleus neurones that project to the rostral ventrolateral medulla is regulated by small-conductance Ca2+-activated K+ channels.The Journal of physiology · 2009Article
- Plasticity and emerging role of BKCa channels in nociceptive control in neuropathic pain.Journal of neurochemistry · 2009Article
- Identification of a BK channel auxiliary protein controlling molecular and behavioral tolerance to alcohol.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Early electrical remodeling in rabbit pulmonary vein results from trafficking of intracellular SK2 channels to membrane sites.Cardiovascular research · 2007Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using in situ hybridisation histochemistry in combination with patch-clamp recordings and specific pharmacological tools, the molecular nature of the channels underlying Ca2+-dependent K+ currents was determined in dorsal vagal neurones (DVNs) of rat brainstem slices. In situ hybridisation analysis at cellular resolution revealed the presence of 'big'-conductance Ca2+- and voltage-activated K+ (BK) channel alpha-subunit mRNA, and of only one 'small'-conductance Ca2+-activated K+ (SK) channel subunit transcript, SK3, at very high levels in DVNs. By contrast, SK1 and SK2 mRNAs were below the threshold limit of detection. The SK channel-mediated after-hyperpolarising current (IAHP) was blocked by apamin with a half-maximal inhibitory concentration of approximately 2.2 nM. This is consistent with homomultimeric SK3 channels mediating IAHP in DVNs. IAHP was also blocked by scyllatoxin (20-30 nM) and curare (100-200 microM). Application of apamin (100 nM) or scyllatoxin (20 nM) invariably caused a substantial increase to 146.1 +/- 10.4 and 181.8 +/- 12.9 % of control, respectively, in the spontaneous firing rate of DVNs. Action potential duration was not affected by these SK channel blockers. The selective BK channel blocker iberiotoxin (50 nM) increased action potential duration by 22.5 +/- 7.3 %, as did low concentrations of tetraethylammonium (0.5 mM; 99.3 +/- 16.4 %) and the Ca2+ channel blocker Cd2+ (100 microM; 49.5 +/- 20.9 %). BK channel blockade did not significantly affect the firing rate of DVNs. These results allow us to establish a tight correlation between the properties of cloned and native BK and SK channels, and to achieve an understanding, at the molecular level, of their role in regulating the spontaneous firing frequency and in shaping single action potentials of central neurones.
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