ArticleJournal of clinical microbiology2000
Molecular and immunological characterization of Mycobacterium tuberculosis CFP-10, an immunodiagnostic antigen missing in Mycobacterium bovis BCG.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 122 citations in OpenAlex.
- Performance of purified antigens for serodiagnosis of pulmonary tuberculosis: a meta-analysis.Clinical and vaccine immunology : CVI · 2009Pooled it
- Genomic Profiling and Mutation Analysis ofMicroorganisms · 2025Article
- Evaluating the Performance of PPE44, HSPX, ESAT-6 and CFP-10 Factors in Tuberculosis Subunit Vaccines.Current microbiology · 2022Review
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- Identification of new diagnostic biomarkers for Mycobacterium tuberculosis and the potential application in the serodiagnosis of human tuberculosis.Microbial biotechnology · 2018Article
- Application of molecular, microbiological, and immunological tests for the diagnosis of bone and joint tuberculosis.Journal of clinical laboratory analysis · 2018Article
- A novel tuberculosis antigen identified from human tuberculosis granulomas.Molecular & cellular proteomics : MCP · 2015Article
- The ESAT-6 protein of Mycobacterium tuberculosis interacts with beta-2-microglobulin (β2M) affecting antigen presentation function of macrophage.PLoS pathogens · 2014Article
- Exploration of novel cellular and serological antigen biomarkers in the ORFeome of Mycobacterium tuberculosis.Molecular & cellular proteomics : MCP · 2014Article
- Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay.Journal of veterinary science · 2014Article
- A neonatal oralTrials in vaccinology · 2013Article
- Antigen-specific CD8(+) T cells and protective immunity to tuberculosis.Advances in experimental medicine and biology · 2013Review
- Serodiagnosis efficacy and immunogenicity of the fusion protein of Mycobacterium tuberculosis composed of the 10-kilodalton culture filtrate protein, ESAT-6, and the extracellular domain fragment of PPE68.Clinical and vaccine immunology : CVI · 2012Article
- Article
- Opportunities for improved serodiagnosis of human tuberculosis, bovine tuberculosis, and paratuberculosis.Veterinary medicine international · 2012Article
- Identification of Mycobacterium tuberculosis antigens of high serodiagnostic value.Clinical and vaccine immunology : CVI · 2010Article
- The CFP-10/ESAT-6 complex of Mycobacterium tuberculosis potentiates the activation of murine macrophages involvement of IFN-gamma signaling.Medical microbiology and immunology · 2010Article
- Immunoproteomic identification of human T cell antigens of Mycobacterium tuberculosis that differentiate healthy contacts from tuberculosis patients.Molecular & cellular proteomics : MCP · 2010Article
- Humoral immune responses against the Mycobacterium tuberculosis 38-kilodalton, MTB48, and CFP-10/ESAT-6 antigens in tuberculosis.Clinical and vaccine immunology : CVI · 2010Article
- Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
In order to identify antigens that may be used in the serodiagnosis of active tuberculosis (TB), we screened a Mycobacterium tuberculosis genomic expression library with a pool of sera from patients diagnosed with active pulmonary TB. The sera used lacked reactivity with a recombinant form of the M. tuberculosis 38-kDa antigen (r38kDa), and the goal was to identify antigens that might complement r38kDa in a serodiagnostic assay. Utilizing this strategy, we identified a gene, previously designated lhp, which encodes a 100-amino-acid protein referred to as culture filtrate protein 10 (CFP-10). The lhp gene is located directly upstream of esat-6, within a region missing in M. bovis BCG. Immunoblot analysis demonstrated that CFP-10 is present in M. tuberculosis CFP, indicating that it is likely a secreted or shed antigen. Purified recombinant CFP-10 (rCFP-10) was shown to be capable of detecting specific antibody in a percentage of TB patients that lack reactivity with r38kDa, most notably in smear-negative cases, where sensitivity was increased from 21% for r38kDa alone to 40% with the inclusion of rCFP-10. In smear-positive patient sera, sensitivity was increased from 49% for r38kDa alone to 58% with the inclusion of rCFP-10. In addition, rCFP-10 was shown to be a potent T-cell antigen, eliciting proliferative responses and gamma interferon production from peripheral blood mononuclear cells in 70% of purified protein derivative-positive individuals without evident disease. The responses to this antigen argue for the inclusion of rCFP-10 in a polyvalent serodiagnostic test for detection of active TB infection. rCFP-10 could also contribute to the development of a recombinant T-cell diagnostic test capable of detecting exposure to M. tuberculosis.
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Registered trials
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