ArticleJournal of clinical microbiology2000
Diagnosis of amebic liver abscess and intestinal infection with the TechLab Entamoeba histolytica II antigen detection and antibody tests.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
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Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it, 254 citations in OpenAlex.
- Fulminant Amebic Colitis after Corticosteroid Therapy: A Systematic Review.PLoS neglected tropical diseases · 2016Pooled it
- A Cross-Sectional Study ofBioMed research international · 2019Trial
- Concomitant Borreliosis and Invasive Amoebiasis: A Rare Case with Suspected Sexual Acquisition.Infectious disease reports · 2026Article
- Ruptured Community-acquired Methicillin-ResistantActa medica Philippina · 2026Article
- Sexually transmitted enteric infections in men who have sex with men.Clinical microbiology reviews · 2025Review
- A case of amebic colitis: A potentially fatal mimicker of inflammatory bowel disease.JPGN reports · 2025Article
- The Impact of a Community Pharmacy-Led Deworming-Related Counselling Service: An Interventional Study in a Low-to-Middle Income Country.Tropical medicine and infectious disease · 2025Article
- A proof-of-concept point-of-care test for the serodiagnosis of human amebic liver abscess.PeerJ · 2025Article
- An Imported Case of Gigantic Amoebic Liver Abscess in a 24-Year-Old Woman in Singapore.Case reports in infectious diseases · 2025Article
- Recent advances in the diagnosis and management of amoebiasis.Frontline gastroenterology · 2025Review
- Evaluation of Molecular Assays for Diagnosis of Amoebic Liver Abscess in India with Bayesian Latent Class Analysis.The American journal of tropical medicine and hygiene · 2024Article
- Amebic liver abscess: An update.World journal of hepatology · 2024Review
- Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples.Scientific reports · 2024Article
- Article
- Liver abscess caused by multidrug-resistant pathogenJournal of family medicine and primary care · 2023Article
- Interleukin 10 (IL-10) Production and Seroprevalence ofPathogens (Basel, Switzerland) · 2022Article
- Diagnostic Methods of Common Intestinal Protozoa: Current and Future Immunological and Molecular Methods.Tropical medicine and infectious disease · 2022Review
- Prevalence and associated risk factors ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Development of a salivary IgA detection method for accurate diagnosis of amebiasis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Accurate laboratory diagnosis of human intestinal and extra-intestinal amoebiasis.Gastroenterology and hepatology from bed to bench · 2022Review
8 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
A noninvasive diagnostic test for amebic liver abscess is needed, because amebic and bacterial abscesses appear identical on ultrasound or computer tomography and because it is rarely possible to identify Entamoeba histolytica in stool specimens from patients with amebic liver abscess. Here we report a method of detection in serum of circulating E. histolytica Gal/GalNAc lectin to diagnose amebic liver abscess, which was used in patients from Dhaka, Bangladesh. The TechLab E. histolytica II test (which differentiates the true pathogen E. histolytica from Entamoeba dispar) detected Gal/GalNAc lectin in the sera of 22 of 23 (96%) amebic liver abscess patients tested prior to treatment with the antiamebic drug metronidazole and 0 of 70 (0%) controls. After 1 week of treatment with metronidazole, 9 of 11 (82%) patients became serum lectin antigen negative. The sensitivity of the E. histolytica II antigen detection test for intestinal infection was also evaluated. Antigen detection identified E. histolytica infection in 50 samples from 1, 164 asymptomatic preschool children aged 2 to 5 years, including 16 of 16 (100%) culture-positive specimens. PCR analysis of stool specimens was used to confirm that most antigen-positive but culture-negative specimens were true-positive: PCR identified parasite DNA in 27 of 34 (79%) of the antigen-positive, culture-negative stool specimens. Antigen detection was a more sensitive test for infection than antilectin antibodies, which were detected in only 76 of 98 (78%) amebic liver abscess patients and in 26 of 50 (52%) patients with intestinal infection. We conclude that the TechLab E. histolytica II kit is a sensitive means to diagnose hepatic and intestinal amebiasis prior to the institution of metronidazole treatment.
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