ArticleJournal of clinical microbiology2000
Phenotypic and genotypic diversity of the flagellin gene (fliC) among Clostridium difficile isolates from different serogroups.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Bioinformatics Analysis and Spatiotemporal Distribution of the fliC Gene and Its Protein Isolated from Escherichia coli-Infected Patients in Eastern Algeria.The Malaysian journal of medical sciences : MJMS · 2024Article
- Understanding host immune responses iniMeta · 2024Review
- Immunization Strategies Against Clostridioides difficile.Advances in experimental medicine and biology · 2024Review
- Recombinant Fusion Protein Vaccine Containing Clostridioides difficile FliC and FliD Protects Mice against C. difficile Infection.Infection and immunity · 2023Article
- Immunoinformatics Approach Toward the Introduction of a Novel Multi-Epitope Vaccine AgainstFrontiers in immunology · 2022Article
- Isolation and characterization of a multidrug-resistantJournal of environmental health science & engineering · 2020Article
- The flagellin of candidate live biotherapeutic Enterococcus gallinarum MRx0518 is a potent immunostimulant.Scientific reports · 2019Article
- A 3D intestinal tissue model supports Clostridioides difficile germination, colonization, toxin production and epithelial damage.Anaerobe · 2018Article
- Article
- Analysis of proteomes released from in vitro cultured eightGut pathogens · 2017Article
- Immunogenicity and protective efficacy of recombinant Clostridium difficile flagellar protein FliC.Emerging microbes & infections · 2016Article
- 'Get in Early'; Biofilm and Wax Moth (Galleria mellonella) Models Reveal New Insights into the Therapeutic Potential of Clostridium difficile Bacteriophages.Frontiers in microbiology · 2016Article
- As Clear as Mud? Determining the Diversity and Prevalence of Prophages in the Draft Genomes of Estuarine Isolates of Clostridium difficile.Genome biology and evolution · 2015Article
- Immune-based treatment and prevention of Clostridium difficile infection.Human vaccines & immunotherapeutics · 2014Review
- The flagellin FliC of Clostridium difficile is responsible for pleiotropic gene regulation during in vivo infection.PloS one · 2014Article
- Clostridium difficile virulence factors: Insights into an anaerobic spore-forming pathogen.Gut microbes · 2014Review
- Genetically diverse Clostridium difficile strains harboring abundant prophages in an estuarine environment.Applied and environmental microbiology · 2013Article
- Clostridium difficile infection in the twenty-first century.Emerging microbes & infections · 2013Review
- The role of flagella in Clostridium difficile pathogenesis: comparison between a non-epidemic and an epidemic strain.PloS one · 2013Article
- Motility and flagellar glycosylation in Clostridium difficile.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenotypic and genotypic diversity of the flagellin gene (fliC) of Clostridium difficile was studied in 47 isolates from various origins belonging to the serogroups A, B, C, D, F, G, H, I, K, X, and S3. Electron microscopy revealed 17 nonflagellated strains and 30 flagellated strains. PCR and reverse transcription-PCR demonstrated that the flagellin gene was present in all strains and that the fliC gene was expressed in both flagellated and nonflagellated strains. Southern blotting showed the presence of only one copy of the gene and three different hybridization patterns. DNA sequence analysis of fliC from the strains belonging to serogroups C, D, and X, representative of each profile, disclosed great variability in the central domain, whereas the N- and C-terminal domains were conserved. The variability of the flagellin gene fliC was further studied in the isolates by PCR-restriction fragment length polymorphism (RFLP) analysis. Nine different RFLP groups were identified (I to IX), among which three (I, VII, and VIII) corresponded to numerous serogroups whereas the six others (II, III, IV, V, VI, and IX) belonged to a single serogroup. Flagellin gene RFLP analysis could constitute an additional typing method employable in conjunction with other typing methods currently available.
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