ArticleBiophysical journal2000
Actin dynamics at the living cell submembrane imaged by total internal reflection fluorescence photobleaching.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 94 citations in OpenAlex.
- Dynamic L-type CaBiochimica et biophysica acta. Molecular cell research · 2018Article
- Fast Focal Point Correction in Prism-Coupled Total Internal Reflection Scanning Imager Using an Electronically Tunable Lens.Sensors (Basel, Switzerland) · 2018Article
- Dances with Membranes: Breakthroughs from Super-resolution Imaging.Current topics in membranes · 2015Review
- High-throughput monitoring of major cell functions by means of lensfree video microscopy.Scientific reports · 2014Article
- Quantification of biomass and cell motion in human pluripotent stem cell colonies.Biophysical journal · 2013Article
- Probing and tracking organelles in living plant cells.Protoplasma · 2012Review
- Rifampicin-independent interactions between the pregnane X receptor ligand binding domain and peptide fragments of coactivator and corepressor proteins.Biochemistry · 2012Article
- Unusually Strong Temperature Dependence of P2X3 Receptor Traffic to the Plasma Membrane.Frontiers in cellular neuroscience · 2011Article
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
- Imaging of dynamic secretory vesicles in living pollen tubes of Picea meyeri using evanescent wave microscopy.Plant physiology · 2006Article
- Nucleoplasmic beta-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations.The Journal of cell biology · 2006Article
- Optical routing and sensing with nanowire assemblies.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Subsecond reorganization of the actin network in cell motility and chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Formation and destabilization of actin filaments with tetramethylrhodamine-modified actin.Biophysical journal · 2004Article
- Detecting rearrangements of shaker and NaChBac in real-time with fluorescence spectroscopy in patch-clamped mammalian cells.Biophysical journal · 2004Article
- The mechanics of neutrophils: synthetic modeling of three experiments.Biophysical journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Although reversible chemistry is crucial to dynamical processes in living cells, relatively little is known about relevant chemical kinetic rates in vivo. Total internal reflection/fluorescence recovery after photobleaching (TIR/FRAP), an established technique previously demonstrated to measure reversible biomolecular kinetic rates at surfaces in vitro, is extended here to measure reversible biomolecular kinetic rates of actin at the cytofacial (subplasma membrane) surface of living cells. For the first time, spatial imaging (with a charge-coupled device camera) is used in conjunction with TIR/FRAP. TIR/FRAP imaging produces both spatial maps of kinetic parameters (off-rates and mobile fractions) and estimates of kinetic correlation distances, cell-wide kinetic gradients, and dependences of kinetic parameters on initial fluorescence intensity. For microinjected rhodamine actin in living cultured smooth muscle (BC3H1) cells, the unbinding rate at or near the cytofacial surface of the plasma membrane (averaged over the entire cell) is measured at 0.032 +/- 0.007 s(-1). The corresponding rate for actin marked by microinjected rhodamine phalloidin is very similar, 0.033 +/- 0.013 s(-1), suggesting that TIR/FRAP is reporting the dynamics of entire filaments or protofilaments. For submembrane fluorescence-marked actin, the intensity, off-rate, and mobile fraction show a positive correlation over a characteristic distance of 1-3 microm and a negative correlation over larger distances greater than approximately 7-14 microm. Furthermore, the kinetic parameters display a statistically significant cell-wide gradient, with the cell having a "fast" and "slow" end with respect to actin kinetics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.