ArticleBiophysical journal2000
Shear-induced assembly of lambda-phage DNA.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Vortex-Flow-Directed Chiral Macroscopic Ordering of Platelet Nanostructures Formed via the Supramolecular Assembly of Platinum Complexes with Bis(phenylisoxazolyl)benzene.Journal of the American Chemical Society · 2025Article
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- The structural role of bacterial eDNA in the formation of biofilm streamers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study.Saudi journal of biological sciences · 2022Article
- Weak and Strong Gels and the Emergence of the Amorphous Solid State.Gels (Basel, Switzerland) · 2018Review
- Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.Journal of clinical microbiology · 2010Article
- Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point.Lab on a chip · 2010Article
- Article
- Shape anisotropy of a single random-walk polymer.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant DNA technology, which is based on the assembly of DNA fragments, forms the backbone of biological and biomedical research. Here we demonstrate that a uniform shear flow can induce and control the assembly of lambda-phage DNA molecules: increasing shear rates form integral DNA multimers of increasing molecular weight. Spontaneous assembly and grouping of end-blunted lambda-phage DNA molecules are negligible. It is suggested that shear-induced DNA assembly is caused by increasing the probability of contact between molecules and by stretching the molecules, which exposes the cohesive ends of the otherwise undeformed lambda-phage DNA molecules. We apply this principle to enhance the kinetics and extent of DNA concatenation in the presence of ligase. This novel approach to controlled DNA assembly could form the basis for improved approaches to gene-chip and recombinant DNA technologies and provide new insight into the rheology of associating polymers.
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