ArticleBiophysical journal2000
Inhibition of Ca(2+) sparks by ruthenium red in permeabilized rat ventricular myocytes.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 74 citations in OpenAlex.
- CaPhysiological reviews · 2024Review
- Dynamin-related protein 1 is a critical regulator of mitochondrial calcium homeostasis during myocardial ischemia/reperfusion injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Anti-Inflammatory Effects of Cannabigerol in Rheumatoid Arthritis Synovial Fibroblasts and Peripheral Blood Mononuclear Cell Cultures Are Partly Mediated by TRPA1.International journal of molecular sciences · 2023Article
- Ryanodine receptor 1-mediated CaJournal of experimental & clinical cancer research : CR · 2022Article
- Magnesium Ions Moderate Calcium-Induced Calcium Release in Cardiac Calcium Release Sites by Binding to Ryanodine Receptor Activation and Inhibition Sites.Frontiers in physiology · 2021Article
- Novel approach for quantification of endoplasmic reticulum CaAmerican journal of physiology. Heart and circulatory physiology · 2019Article
- Unique CaInternational journal of molecular sciences · 2018Review
- Structure-function relationships of peptides forming the calcin family of ryanodine receptor ligands.The Journal of general physiology · 2016Article
- Regulation of calcium clock-mediated pacemaking by inositol-1,4,5-trisphosphate receptors in mouse sinoatrial nodal cells.The Journal of physiology · 2015Article
- Ca²⁺-dependent phosphorylation of Ca²⁺ cycling proteins generates robust rhythmic local Ca²⁺ releases in cardiac pacemaker cells.Science signaling · 2013Article
- A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.PloS one · 2013Article
- Luminal Ca2+ controls activation of the cardiac ryanodine receptor by ATP.The Journal of general physiology · 2012Article
- Construction of calcium release sites in cardiac myocytes.Frontiers in physiology · 2012Article
- How does stochastic ryanodine receptor-mediated Ca leak fail to initiate a Ca spark?Biophysical journal · 2011Article
- Differential sensitivity of Ca²+ wave and Ca²+ spark events to ruthenium red in isolated permeabilised rabbit cardiomyocytes.The Journal of physiology · 2010Article
- Ca²+ spark-dependent and -independent sarcoplasmic reticulum Ca²+ leak in normal and failing rabbit ventricular myocytes.The Journal of physiology · 2010Article
- Frequency and release flux of calcium sparks in rat cardiac myocytes: a relation to RYR gating.The Journal of general physiology · 2010Article
- Mitochondria in cardiomyocyte Ca2+ signaling.The international journal of biochemistry & cell biology · 2009Review
- The transient receptor potential vanilloid-1 channel in thermoregulation: a thermosensor it is not.Pharmacological reviews · 2009Review
- Ryanodine receptor-mediated arrhythmias and sudden cardiac death.Pharmacology & therapeutics · 2009Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have compared the effects of the sarcoplasmic reticulum (SR) Ca(2+) release inhibitor, ruthenium red (RR), on single ryanodine receptor (RyR) channels in lipid bilayers, and on Ca(2+) sparks in permeabilized rat ventricular myocytes. Ruthenium red at 5 microM inhibited the open probability (P(o)) of RyRs approximately 20-50-fold, without significantly affecting the conductance or mean open time of the channel. At the same concentration, RR inhibited the frequency of Ca(2+) sparks in permeabilized myocytes by approximately 10-fold, and reduced the amplitude of large amplitude events (with most probable localization on the line scan) by approximately 3-fold. According to our theoretical simulations, performed with a numerical model of Ca(2+) spark formation, this reduction in Ca(2+) spark amplitude corresponds to an approximately 4-fold decrease in Ca(2+) release flux underlying Ca(2+) sparks. Ruthenium red (5 microM) increased the SR Ca(2+) content by approximately 2-fold (from 151 to 312 micromol/l cytosol). Considering the degree of inhibition of local Ca(2+) release events, the increase in SR Ca(2+) load by RR, and the lack of effects of RR on single RyR open time and conductance, we have estimated that Ca(2+) sparks under normal conditions are generated by openings of at least 10 single RyRs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.