ArticleInvestigative ophthalmology & visual science2000
Expression of betaB(2)-crystallin mRNA and protein in retina, brain, and testis.
Article in Investigative ophthalmology & visual science, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Physiological and pathological functions of βB2-crystallins in multiple organs: a systematic review.Aging · 2021Pooled it
- Quantitative spatial analysis of crystallin proteins in human lens epithelial cells.Scientific reports · 2025Article
- ATCUN-like Copper Site in βB2-Crystallin Plays a Protective Role in Cataract-Associated Aggregation.Inorganic chemistry · 2023Article
- Broadening the Mutation Spectrum inFrontiers in medicine · 2021Article
- Mouse models for microphthalmia, anophthalmia and cataracts.Human genetics · 2019Review
- Crybb2 Mutations Consistently Affect Schizophrenia Endophenotypes in Mice.Molecular neurobiology · 2019Article
- Mutations in crystallin genes result in congenital cataract associated with other ocular abnormalities.Molecular vision · 2017Article
- Transcriptome Analysis and Differential Gene Expression on the Testis of Orange Mud Crab, Scylla olivacea, during Sexual Maturation.PloS one · 2017Article
- Long non-coding RNAs regulate effects of β-crystallin B2 on mouse ovary development.Molecular medicine reports · 2016Article
- A NovelJournal of ophthalmology · 2016Article
- βA3/A1-crystallin: more than a lens protein.Progress in retinal and eye research · 2015Review
- Functions of crystallins in and out of lens: roles in elongated and post-mitotic cells.Progress in biophysics and molecular biology · 2014Review
- Clinical and experimental advances in congenital and paediatric cataracts.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Light-induced phosphorylation of crystallins in the retinal pigment epithelium.International journal of biological macromolecules · 2011Article
- The transcription factor Pax6 regulates survival of dopaminergic olfactory bulb neurons via crystallin αA.Neuron · 2010Article
- Molecular analysis of cataract families in India: new mutations in the CRYBB2 and GJA3 genes and rare polymorphisms.Molecular vision · 2010Article
- Mouse models of cataract.Journal of genetics · 2009Review
- Congenital cataracts and their molecular genetics.Seminars in cell & developmental biology · 2008Review
- Mutation analysis in a German family identified a new cataract-causing allele in the CRYBB2 gene.Molecular vision · 2007Article
- Subfertility in mice harboring a mutation in betaB2-crystallin.Molecular vision · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo evaluate the expression of betaB(2)-crystallin mRNA and protein in rat, bovine, and human nonlens and nonocular tissues.
methodsbetaB(2)-crystallin mRNA levels were detected by RT-PCR. betaB(2)-crystallin protein was purified from rat and bovine tissues by FPLC chromatography. FPLC fractions were analyzed by immunoblotting. The identity of betaB(2)-crystallin protein, isolated from the retina, was confirmed by protein microsequencing.
resultsbetaB(2)-crystallin transcript was detected in rat brain, rat testis, and human retina by RT-PCR. betaB(2)-crystallin transcript was not found in rat lung, heart, ovary, spleen, thymus, kidney, and liver or in human brain and testis. betaB(2)-crystallin protein was partially purified from and its identity confirmed in rat brain, rat testis, and bovine retina. The bovine retinal protein was further confirmed to be authentic betaB(2)-crystallin by protein microsequencing.
conclusionsThese results establish that betaB(2)-crystallin mRNA and protein are expressed in tissues outside of the lens and outside of the eye including retina, brain, and testis. Extralenticular and extraocular expression of betaB(2)-crystallin, coupled with its participation in phosphorylation pathways, suggests that it has nonrefractive functions in these tissues.
Indexed as
Identifiers
10967064PMC2825739W1780144148What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.