ArticleApplied and environmental microbiology2000
Application of sequence-specific labeled 16S rRNA gene oligonucleotide probes for genetic profiling of cyanobacterial abundance and diversity by array hybridization.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 109 citations in OpenAlex.
- Genetic divergence among toxic and non-toxic cyanobacteria of the dry zone of Sri Lanka.SpringerPlus · 2016Article
- Specific discrimination of three pathogenic Salmonella enterica subsp. enterica serotypes by carB-based oligonucleotide microarray.Applied and environmental microbiology · 2014Article
- Phylogeny and biogeography of cyanobacteria and their produced toxins.Marine drugs · 2013Review
- Identification and enumeration of Microcystis using a sandwich hybridization assay.Journal of microbiology (Seoul, Korea) · 2012Article
- Evaluation of single-nucleotide primer extension for detection and typing of phylogenetic markers used for investigation of microbial communities.Applied and environmental microbiology · 2009Article
- Microarray applications in microbial ecology research.Microbial ecology · 2006Review
- Technology development to explore the relationship between oral health and the oral microbial community.BMC oral health · 2006Article
- Development and applications of microbial ecogenomic indicators for monitoring water quality: report of a workshop assessing the state of the science, research needs and future directions.Environmental monitoring and assessment · 2006Article
- Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria.Applied and environmental microbiology · 2005Article
- Development of a new oligonucleotide array to identify staphylococcal strains at species level.Journal of clinical microbiology · 2005Article
- Development of a universal microarray based on the ligation detection reaction and 16S rrna gene polymorphism to target diversity of cyanobacteria.Applied and environmental microbiology · 2004Article
- Evidence for multiple lateral transfers of the circadian clock cluster in filamentous heterocystic cyanobacteria Nostocaceae.Journal of molecular evolution · 2004Article
- Direct real-time PCR quantification of Campylobacter jejuni in chicken fecal and cecal samples by integrated cell concentration and DNA purification.Applied and environmental microbiology · 2004Article
- Optimization strategies for DNA microarray-based detection of bacteria with 16S rRNA-targeting oligonucleotide probes.Applied and environmental microbiology · 2003Article
- Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction).BMC microbiology · 2002Article
- PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database.Nucleic acids research · 2002Article
- Analysis of bacterial community composition by oligonucleotide fingerprinting of rRNA genes.Applied and environmental microbiology · 2002Article
- Development and evaluation of a 16S ribosomal DNA array-based approach for describing complex microbial communities in ready-to-eat vegetable salads packed in a modified atmosphere.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA sequence information for the small-subunit rRNA gene (16S rDNA) obtained from cyanobacterial cultures was used to investigate the presence of cyanobacteria and their abundance in natural habitats. Eight planktonic communities developing in lakes characterized by relatively low algal biomass (mesotrophic) and in lakes with correspondingly high biomass (eutrophic) were selected for the study. The organismal compositions of the water samples were analyzed genetically, using multiplex sequence-specific labeling of oligonucleotide probes targeted to 16S rDNA and subsequent hybridization of the labeled probes to their respective complements spotted onto a solid support (DNA array). Ten probes were established to determine the relative abundances of the discernible cyanobacteria encountered in the selected lakes. The probes were generally specific for their targets, as determined through analyses of clone cultures. Reproducible abundance profiles were established for the lakes investigated in the subsequent analyses of natural cyanobacterial communities. The results from the genetic analyses were then compared with information obtained from standard hydrobiological and hydrochemical analyses. Qualitatively, there were relatively good correlations among the groups of organisms (Nostoc, Microcystis, and Planktothrix species) found in the different lakes. The levels of correlation were lower for the quantitative data. This may, however, be due to differences in sample processing technique. The conclusions from these comparisons are that the genetic abundance profiles may provide a foundation for separating and quantifying genetically distinct groups of cyanobacteria in their natural habitats.
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