ArticleApplied and environmental microbiology2000
Development of a fluorogenic 5' nuclease PCR assay for detection of the ail gene of pathogenic Yersinia enterocolitica.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 73 citations in OpenAlex.
- Multiplex PCR method for differentiating highly pathogenic Yersinia enterocolitica and low pathogenic Yersinia enterocolitica, and Yersinia pseudotuberculosis.The Journal of veterinary medical science · 2021Article
- Yersiniosis in New Zealand.Pathogens (Basel, Switzerland) · 2021Review
- Selection of potential aptamers for specific growth stage detection ofRSC advances · 2020Article
- Detection of Yersinia enterocolitica in food: an overview.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Review
- Presence of Salmonella spp., Yersinia enterocolitica, Yersinia pseudotuberculosis and Escherichia coli O157:H7 in wild boars.Epidemiology and infection · 2014Article
- Real-time TaqMan PCR for Yersinia enterocolitica detection based on the ail and foxA genes.Journal of clinical microbiology · 2014Article
- Specific identification of pathogenic Yersinia enterocolitica by monoclonal antibodies generated against recombinant attachment invasion locus (rAil) protein.World journal of microbiology & biotechnology · 2012Article
- Real-time PCR analysis of enteric pathogens from fecal samples of irritable bowel syndrome subjects.Gut pathogens · 2011Article
- Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods.Journal of pathogens · 2011Article
- Possible use of ail and foxA polymorphisms for detecting pathogenic Yersinia enterocolitica.BMC microbiology · 2010Article
- Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food.Applied and environmental microbiology · 2008Article
- Prevalence of pathogenic Yersinia enterocolitica strains in pigs in the United States.Applied and environmental microbiology · 2005Article
- Low occurrence of pathogenic Yersinia enterocolitica in clinical, food, and environmental samples: a methodological problem.Clinical microbiology reviews · 2003Review
- Modeling of 5' nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica.Journal of clinical microbiology · 2002Article
- Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report we describe the development and evaluation of a fluorogenic PCR assay for the detection of pathogenic Yersinia enterocolitica. The assay targets the chromosomally encoded attachment and invasion gene, ail. Three primer-probe sets (TM1, TM2, and TM3) amplifying different, yet overlapping, regions of ail were examined for their specificity and sensitivity. All three primer-probe sets were able to detect between 0.25 and 0.5 pg of purified Y. enterocolitica DNA. TM1 identified all 26 Y. enterocolitica strains examined. TM3 was able to detect all strains except one, whereas TM2 was unable to detect 10 of the Y. enterocolitica strains tested. None of the primer-probe sets cross-reacted with any of the 21 non-Y. enterocolitica strains examined. When the TM1 set was utilized, the fluorogenic PCR assay was able to detect </=4 Y. enterocolitica CFU/ml in pure culture and 10 Y. enterocolitica CFU/ml independent of the presence of 10(8) CFU of contaminating bacteria per ml. This set was also capable of detecting </=1 CFU of Y. enterocolitica per g of ground pork or feces after a 24-h enrichment in a Yersinia selective broth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.