ArticleProceedings of the National Academy of Sciences of the United States of America2000
Virus neutralization by germ-line vs. hypermutated antibodies.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- MF59 adjuvant enhances diversity and affinity of antibody-mediated immune response to pandemic influenza vaccines.Science translational medicine · 2011Trial
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- The dengue-specific immune response and antibody identification with machine learning.NPJ vaccines · 2024Article
- PD-1Cell reports · 2023Article
- Potent germline-like monoclonal antibodies: rapid identification of promising candidates for antibody-based antiviral therapy.Antibody therapeutics · 2021Article
- Article
- Deep Sequencing of B Cell Receptor Repertoires From COVID-19 Patients Reveals Strong Convergent Immune Signatures.Frontiers in immunology · 2020Article
- A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus.PLoS neglected tropical diseases · 2017Article
- Lower IgG somatic hypermutation rates during acute dengue virus infection is compatible with a germinal center-independent B cell response.Genome medicine · 2016Article
- How specific is too specific? B-cell responses to viral infections reveal the importance of breadth over depth.Immunological reviews · 2013Review
- Article
- Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Use of molecular modeling and site-directed mutagenesis to define the structural basis for the immune response to carbohydrate xenoantigens.BMC immunology · 2007Article
- Polyreactive antigen-binding B (PAB-) cells are widely distributed and the PAB population consists of both B-1+ and B-1- phenotypes.Clinical and experimental immunology · 2004Article
- Construction and functional activities of chimeric mouse-human immunoglobulin G and immunoglobulin M antibodies against the Neisseria meningitidis PorA P1.7 and P1.16 epitopes.Infection and immunity · 2003Article
- Molecular features of the broadly neutralizing immunoglobulin G1 b12 required for recognition of human immunodeficiency virus type 1 gp120.Journal of virology · 2003Article
- Specificity and diversity of antibodies to Mycobacterium tuberculosis arabinomannan.Clinical and diagnostic laboratory immunology · 2003Article
- Neutralizing antiviral antibody responses.Advances in immunology · 2001Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mice infected with vesicular stomatitis virus (VSV), a cytopathic virus closely related to rabies virus, mount a virus-neutralizing antibody response protecting against lethal disease. VSVneutralizing monoclonal IgGs isolated from primary immune responses were devoid of somatic mutations, whereas most secondary and all hyperimmune response IgGs tested were hypermutated. A comparative analysis of recombinant single-chain antibody fragments (scFv-Ckappa) revealed that even the germ-line precursor of one hypermutated antibody bound and neutralized VSV. Four somatic amino acid substitutions in V(H) increased by 300-fold the binding strength of monovalent scFv-Ckappa. The multivalent binding avidity of germ-line scFv-Ckappa was increased by more than 10-fold compared with the monovalent binding strength. In contrast, hypermutated scFv-Ckappa did not show such avidity effects. Thus the overall binding difference between the germ-line and the hypermutated VSV-neutralizing antibody was only 10- to 15-fold. This may explain why primary germ-line antibodies and secondary hypermutated antibodies directed against pathogens such as viruses and bacteria expressing repetitive antibody determinants show rather similar binding qualities, whereas monovalently binding hapten-specific antibodies can show "affinity maturation" effects of up to 1000-fold.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.