ArticleProceedings of the National Academy of Sciences of the United States of America2000
How well can an amoeba climb?
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
- A Centrifuge Polarizing Microscope (CPM) That Enables the Visualization of Intracellular Structures Under High Centrifugal Forces.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in thebioRxiv : the preprint server for biology · 2024Article
- Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces.Materials (Basel, Switzerland) · 2020Article
- Myosin II is essential for the spatiotemporal organization of traction forces during cell motility.Molecular biology of the cell · 2010Article
- Distribution of traction forces associated with shape changes during amoeboid cell migration.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2009Article
- Spatio-temporal analysis of eukaryotic cell motility by improved force cytometry.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The role of myosin II motor activity in distributing myosin asymmetrically and coupling protrusive activity to cell translocation.Molecular biology of the cell · 2006Article
- Asymmetric distribution of myosin IIB in migrating endothelial cells is regulated by a rho-dependent kinase and contributes to tail retraction.Molecular biology of the cell · 2003Article
- Dictyostelium discoideum adhesion and motility under shear flow: experimental and theoretical approaches.Journal of muscle research and cell motility · 2002Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here our efforts to measure the crawling force generated by cells undergoing amoeboid locomotion. In a centrifuge microscope, acceleration was increased until amoebae of Dictyostelium discoideum were "stalled" or no longer able to "climb up." The "apparent weight" of the amoebae at stalling rpm in myosin mutants depended on the presence of myosin II (but not myosins IA and IB) and paralleled the cortical strength of the cells. Surprisingly, however, the cell stalled not only in low-density media as expected but also in media with densities greater than the cell density where the buoyant force should push the amoeba upward. We find that the leading pseudopod is bent under centrifugal force in all stalled amoebae, suggesting that this pseudopod is very dense indeed. This finding also suggests that directional cell locomotion against resistive forces requires a turgid forward-pointing pseudopod, most likely sustained by cortical actomyosin II.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.