ArticleProceedings of the National Academy of Sciences of the United States of America2000
Anterograde flow of cargo across the golgi stack potentially mediated via bidirectional "percolating" COPI vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 113 citations in OpenAlex.
- Models of Intracellular Transport: Contradictions and Current Understanding.Sub-cellular biochemistry · 2026Review
- The mechanistic basis of cargo selection during Golgi maturation.Science advances · 2025Article
- Targeting COPA to Enhance Erdafitinib Sensitivity in FGFR-Altered Bladder Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- STED imaging of endogenously tagged ARF GTPases reveals their distinct nanoscale localizations.The Journal of cell biology · 2023Article
- Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra-Golgi recycling vesicles.Traffic (Copenhagen, Denmark) · 2023Article
- Intracellular transport dynamics revealed by single-particle tracking.Biophysics reports · 2021Article
- Means of intracellular communication: touching, kissing, fusing.Microbial cell (Graz, Austria) · 2021Article
- Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.The Journal of cell biology · 2019Article
- A Kinetic View of Membrane Traffic Pathways Can Transcend the Classical View of Golgi Compartments.Frontiers in cell and developmental biology · 2019Review
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
- Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation.Alcoholism, clinical and experimental research · 2016Article
- COPI is essential for Golgi cisternal maturation and dynamics.Journal of cell science · 2016Article
- Rab43 regulates the sorting of a subset of membrane protein cargo through the medial Golgi.Molecular biology of the cell · 2016Article
- Bridging the Gap between Glycosylation and Vesicle Traffic.Frontiers in cell and developmental biology · 2016Review
- Article
- The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival.The Journal of biological chemistry · 2014Article
- Golgi compartmentation and identity.Current opinion in cell biology · 2014Review
- Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.eLife · 2014Article
- Article
- Golgi's way: a long path toward the new paradigm of the intra-Golgi transport.Histochemistry and cell biology · 2013Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How do secretory proteins and other cargo targeted to post-Golgi locations traverse the Golgi stack? We report immunoelectron microscopy experiments establishing that a Golgi-restricted SNARE, GOS 28, is present in the same population of COPI vesicles as anterograde cargo marked by vesicular stomatitis virus glycoprotein, but is excluded from the COPI vesicles containing retrograde-targeted cargo (marked by KDEL receptor). We also report that GOS 28 and its partnering t-SNARE heavy chain, syntaxin 5, reside together in every cisterna of the stack. Taken together, these data raise the possibility that the anterograde cargo-laden COPI vesicles, retained locally by means of tethers, are inherently capable of fusing with neighboring cisternae on either side. If so, quanta of exported proteins would transit the stack in GOS 28-COPI vesicles via a bidirectional random walk, entering at the cis face and leaving at the trans face and percolating up and down the stack in between. Percolating vesicles carrying both post-Golgi cargo and Golgi residents up and down the stack would reconcile disparate observations on Golgi transport in cells and in cell-free systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.