ArticleJournal of bacteriology2000
Characterization of vibrio cholerae O1 antigen as the bacteriophage K139 receptor and identification of IS1004 insertions aborting O1 antigen biosynthesis.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 60 citations in OpenAlex.
- Combined Forces Against Bacteria: Phages and Antibiotics.Health science reports · 2025Article
- Role of Bacteriophages in the Evolution of Pathogenic Vibrios and Lessons for Phage Therapy.Advances in experimental medicine and biology · 2023Article
- Spontaneous Resistance ofFrontiers in microbiology · 2022Article
- Interactions between the Prophage 919TP and ItsViruses · 2021Article
- Adaptive Synthesis of a Rough Lipopolysaccharide in Geobacter sulfurreducens for Metal Reduction and Detoxification.Applied and environmental microbiology · 2021Article
- Phenotypic and Genotypic Properties ofMicroorganisms · 2020Article
- Broadscale phage therapy is unlikely to select for widespread evolution of bacterial resistance to virus infection.Virus evolution · 2020Article
- Functional Specialization inmBio · 2019Article
- Vibrio cholerae motility exerts drag force to impede attack by the bacterial predator Bdellovibrio bacteriovorus.Nature communications · 2018Article
- Review
- The Resistance of Vibrio cholerae O1 El Tor Strains to the Typing Phage 919TP, a Member of K139 Phage Family.Frontiers in microbiology · 2016Article
- Core lipopolysaccharide-specific phage SSU5 as an Auxiliary Component of a Phage Cocktail for Salmonella biocontrol.Applied and environmental microbiology · 2014Article
- Vibrio vulnificus bacteriophage SSP002 as a possible biocontrol agent.Applied and environmental microbiology · 2014Article
- Outer membrane vesicles mediate transport of biologically active Vibrio cholerae cytolysin (VCC) from V. cholerae strains.PloS one · 2014Article
- O antigen is the receptor of Vibrio cholerae serogroup O1 El Tor typing phage VP4.Journal of bacteriology · 2013Article
- The novel virulence-related gene nlxA in the lipopolysaccharide cluster of Xanthomonas citri ssp. citri is involved in the production of lipopolysaccharide and extracellular polysaccharide, motility, biofilm formation and stress resistance.Molecular plant pathology · 2012Article
- Identification of the lipopolysaccharide core of Yersinia pestis and Yersinia pseudotuberculosis as the receptor for bacteriophage φA1122.Journal of bacteriology · 2011Article
- The wxacO gene of Xanthomonas citri ssp. citri encodes a protein with a role in lipopolysaccharide biosynthesis, biofilm formation, stress tolerance and virulence.Molecular plant pathology · 2011Article
- Genetic diversity of O-antigen biosynthesis regions in Vibrio cholerae.Applied and environmental microbiology · 2011Article
- The ColR/ColS two-component system plays multiple roles in the pathogenicity of the citrus canker pathogen Xanthomonas citri subsp. citri.Journal of bacteriology · 2011Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacteriophage K139 was recently characterized as a temperate phage of O1 Vibrio cholerae. In this study we have determined the phage adsorption site on the bacterial cell surface. Phage-binding studies with purified lipopolysaccharide (LPS) of different O1 serotypes and biotypes revealed that the O1 antigen serves as the phage receptor. In addition, phage-resistant O1 El Tor strains were screened by using a virulent isolate of phage K139. Analysis of the LPS of such spontaneous phage-resistant mutants revealed that most of them synthesize incomplete LPS molecules, composed of either defective O1 antigen or core oligosaccharide. By applying phage-binding studies, it was possible to distinguish between receptor mutants and mutations which probably caused abortion of later steps of phage infection. Furthermore, we investigated the genetic nature of O1-negative strains by Southern hybridization with probes specific for the O antigen biosynthesis cluster (rfb region). Two of the investigated O1 antigen-negative mutants revealed insertions of element IS1004 into the rfb gene cluster. Treating one wbeW::IS1004 serum-sensitive mutant with normal human serum, we found that several survivors showed precise excision of IS1004, restoring O antigen biosynthesis and serum resistance. Investigation of clinical isolates by screening for phage resistance and performing LPS analysis of nonlysogenic strains led to the identification of a strain with decreased O1 antigen presentation. This strain had a significant reduction in its ability to colonize the mouse small intestine.
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