ArticleMolecular and cellular biology2000
A homolog of voltage-gated Ca(2+) channels stimulated by depletion of secretory Ca(2+) in yeast.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 1 of them a synthesis that pooled it.
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Who cites it
92 citing papers in PubMed, 1 synthesis or guideline pooled it, 216 citations in OpenAlex.
- Transcriptomic meta-analysis to identify potential antifungal targets in Candida albicans.BMC microbiology · 2024Pooled it
- Calcium starvation leads to strain-specific gene regulation of lipid and carotenoid production in Mucor circinelloides.G3 (Bethesda, Md.) · 2025Article
- KCS1 and VIP1, the genes encoding yeast phosphoinositol pyrophosphate synthases, are required for CaFEBS open bio · 2025Article
- Chemical and Genetic Validation of an Essential Calcium Entry Channel ofACS infectious diseases · 2025Article
- Calcium Signaling Is a Universal Carbon Source Signal Transducer and Effects an Ionic Memory of Past Carbon Sources.International journal of molecular sciences · 2025Article
- High-Affinity Plasma Membrane CaInternational journal of molecular sciences · 2024Article
- Golgi apparatus-localized CATION CALCIUM EXCHANGER4 promotes osmotolerance of Arabidopsis.Plant physiology · 2024Article
- Modeling Calcium Signaling inFrontiers in molecular biosciences · 2022Article
- Molecular Biology of Cadmium Toxicity in Saccharomyces cerevisiae.Biological trace element research · 2021Review
- With an Ear Up against the Wall: An Update on Mechanoperception inPlants (Basel, Switzerland) · 2021Review
- Cation Transporters ofBiomolecules · 2021Review
- Origins of eukaryotic excitability.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Highly conserved extracellular residues mediate interactions between pore-forming and regulatory subunits of the yeast CaThe Journal of biological chemistry · 2020Article
- Stable and destabilized GFP reporters to monitor calcineurin activity inMicrobial cell (Graz, Austria) · 2020Article
- Characterization of Hailey-Hailey Disease-mutants in presence and absence of wild type SPCA1 using Saccharomyces cerevisiae as model organism.Scientific reports · 2019Article
- Article
- Possible Role of the CaMolecules (Basel, Switzerland) · 2019Article
- Increased mitochondrial respiration promotes survival from endoplasmic reticulum stress.Cell death and differentiation · 2019Article
- Calcium Transport Proteins in Fungi: The Phylogenetic Diversity of Their Relevance for Growth, Virulence, and Stress Resistance.Frontiers in microbiology · 2019Review
- Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations.Frontiers in physiology · 2018Article
32 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In animal cells, capacitative calcium entry (CCE) mechanisms become activated specifically in response to depletion of calcium ions (Ca(2+)) from secretory organelles. CCE serves to replenish those organelles and to enhance signaling pathways that respond to elevated free Ca(2+) concentrations in the cytoplasm. The mechanism of CCE regulation is not understood because few of its essential components have been identified. We show here for the first time that the budding yeast Saccharomyces cerevisiae employs a CCE-like mechanism to refill Ca(2+) stores within the secretory pathway. Mutants lacking Pmr1p, a conserved Ca(2+) pump in the secretory pathway, exhibit higher rates of Ca(2+) influx relative to wild-type cells due to the stimulation of a high-affinity Ca(2+) uptake system. Stimulation of this Ca(2+) uptake system was blocked in pmr1 mutants by expression of mammalian SERCA pumps. The high-affinity Ca(2+) uptake system was also stimulated in wild-type cells overexpressing vacuolar Ca(2+) transporters that competed with Pmr1p for substrate. A screen for yeast mutants specifically defective in the high-affinity Ca(2+) uptake system revealed two genes, CCH1 and MID1, previously implicated in Ca(2+) influx in response to mating pheromones. Cch1p and Mid1p were localized to the plasma membrane, coimmunoprecipitated from solubilized membranes, and shown to function together within a single pathway that ensures that adequate levels of Ca(2+) are supplied to Pmr1p to sustain secretion and growth. Expression of Cch1p and Mid1p was not affected in pmr1 mutants. The evidence supports the hypothesis that yeast maintains a homeostatic mechanism related to CCE in mammalian cells. The homology between Cch1p and the catalytic subunit of voltage-gated Ca(2+) channels raises the possibility that in some circumstances CCE in animal cells may involve homologs of Cch1p and a conserved regulatory mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.