ArticleMolecular and cellular biology2000
Preferential accessibility of the yeast his3 promoter is determined by a general property of the DNA sequence, not by specific elements.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Chromosome organization in bacteria: mechanistic insights into genome structure and function.Nature reviews. Genetics · 2020Review
- Modulation of nucleosomal DNA accessibility via charge-altering post-translational modifications in histone core.Epigenetics & chromatin · 2018Article
- Spurious transcription and its impact on cell function.Transcription · 2018Article
- Mechanisms by which transcription factors gain access to target sequence elements in chromatin.Current opinion in genetics & development · 2013Review
- Article
- Nucleosome depletion at yeast terminators is not intrinsic and can occur by a transcriptional mechanism linked to 3'-end formation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Article
- Histone modifications, but not nucleosomal positioning, correlate with major histocompatibility complex class I promoter activity in different tissues in vivo.Molecular and cellular biology · 2008Article
- DNA physical properties determine nucleosome occupancy from yeast to fly.Nucleic acids research · 2008Article
- A nucleosome-guided map of transcription factor binding sites in yeast.PLoS computational biology · 2007Article
- Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection.Genome research · 2006Article
- Activation of Saccharomyces cerevisiae HIS3 results in Gcn4p-dependent, SWI/SNF-dependent mobilization of nucleosomes over the entire gene.Molecular and cellular biology · 2006Article
- Effect of sequence-directed nucleosome disruption on cell-type-specific repression by alpha2/Mcm1 in the yeast genome.Eukaryotic cell · 2006Article
- Flexible promoter architecture requirements for coactivator recruitment.BMC molecular biology · 2006Article
- Dimethylation of histone H3 at lysine 36 demarcates regulatory and nonregulatory chromatin genome-wide.Molecular and cellular biology · 2005Article
- Genomic analysis of LexA binding reveals the permissive nature of the Escherichia coli genome and identifies unconventional target sites.Genes & development · 2005Article
- ATG deserts define a novel core promoter subclass.Genome research · 2005Article
- Improved alignment of nucleosome DNA sequences using a mixture model.Nucleic acids research · 2005Article
- Eaf3 regulates the global pattern of histone acetylation in Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Yeast promoter regions are often more accessible to nuclear proteins than are nonpromoter regions. As assayed by HinfI endonuclease cleavage in living yeast cells, HinfI sites located in the promoters of all seven genes tested were 5- to 20-fold more accessible than sites in adjacent nonpromoter regions. HinfI hypersensitivity within the his3 promoter region is locally determined, since it was observed when this region was translocated to the middle of the ade2 structural gene. Detailed analysis of the his3 promoter indicated that preferential accessibility is not determined by specific elements such as the Gcn4 binding site, poly(dA-dT) sequences, TATA elements, or initiator elements or by transcriptional activity. However, progressive deletion of the promoter region in either direction resulted in a progressive loss of HinfI accessibility. Preferential accessibility is independent of the Swi-Snf chromatin remodeling complex, Gcn5 histone acetylase complexes Ada and SAGA, and Rad6, which ubiquitinates histone H2B. These results suggest that preferential accessibility of the his3 (and presumably other) promoter regions is determined by a general property of the DNA sequence (e.g., base composition or a related feature) rather than by defined sequence elements. The organization of the compact yeast genome into inherently distinct promoter and nonpromoter regions may ensure that transcription factors bind preferentially to appropriate sites in promoters rather than to the excess of irrelevant but equally high-affinity sites in nonpromoter regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.