ArticleMolecular and cellular biology2000
trans splicing of polycistronic Caenorhabditis elegans pre-mRNAs: analysis of the SL2 RNA.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans.Bioscience reports · 2024Article
- SLIDR and SLOPPR: flexible identification of spliced leader trans-splicing and prediction of eukaryotic operons from RNA-Seq data.BMC bioinformatics · 2021Article
- Resolution of polycistronic RNA by SL2RNA (New York, N.Y.) · 2020Article
- Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni.Scientific reports · 2018Article
- An in vivo genetic screen for genes involved in spliced leader trans-splicing indicates a crucial role for continuous de novo spliced leader RNP assembly.Nucleic acids research · 2017Article
- On the Possibility of an Early Evolutionary Origin for the Spliced Leader Trans-Splicing.Journal of molecular evolution · 2017Review
- Inter-organ signalling by HRG-7 promotes systemic haem homeostasis.Nature cell biology · 2017Article
- Article
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Origin and evolution of spliceosomal introns.Biology direct · 2012Review
- Polycistronic pre-mRNA processing in vitro: snRNP and pre-mRNA role reversal in trans-splicing.Genes & development · 2010Article
- SL2-like spliced leader RNAs in the basal nematode Prionchulus punctatus: New insight into the evolution of nematode SL2 RNAs.RNA (New York, N.Y.) · 2010Article
- In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs.BMC molecular biology · 2008Article
- Identification and analysis of internal promoters in Caenorhabditis elegans operons.Genome research · 2007Article
- Profiling Caenorhabditis elegans non-coding RNA expression with a combined microarray.Nucleic acids research · 2006Article
- Identification and analysis of U5 snRNA variants in Drosophila.RNA (New York, N.Y.) · 2005Article
- A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons.Genes & development · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Genes in Caenorhabditis elegans operons are transcribed as polycistronic pre-mRNAs in which downstream gene products are trans spliced to a specialized spliced leader, SL2. SL2 is donated by a 110-nucleotide RNA, SL2 RNA, present in the cell as an Sm-bound snRNP. SL2 RNA can be conceptually folded into a phylogenetically conserved three-stem-loop secondary structure. Here we report an in vivo mutational analysis of the SL2 RNA. Some sequences can be changed without consequence, while other changes result in a substantial loss of trans splicing. Interestingly, the spliced leader itself can be dramatically altered, such that the first stem-loop cannot form, with only a relatively small loss in trans-splicing efficiency. However, the primary sequence of stem II is crucial for SL2 trans splicing. Similarly, the conserved primary sequence of the third stem-loop plays a key role in trans splicing. While mutations in stem-loop III allow snRNP formation, a single nucleotide substitution in the loop prevents trans splicing. In contrast, the analogous region of SL1 RNA is not highly conserved, and its mutation does not abrogate function. Thus, stem-loop III appears to confer a specific function to SL2 RNA. Finally, an upstream sequence, previously predicted to be a proximal sequence element, is shown to be required for SL2 RNA expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.