ArticleProceedings of the National Academy of Sciences of the United States of America2000
Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 94 citations in OpenAlex.
- JAGGED1 modulates bull spermatozoa acrosome reaction and fertilization.Reproduction & fertility · 2026Article
- ATPase N-ethylmaleimide-sensitive factor mediated calcium dependent fusion pore closure and endocytosis.Communications biology · 2026Article
- Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm.Frontiers in cell and developmental biology · 2024Review
- Discrete Dynamic Model of the Mammalian Sperm Acrosome Reaction: The Influence of Acrosomal pH and Physiological Heterogeneity.Frontiers in physiology · 2021Article
- Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.PloS one · 2015Article
- Sustained high protein-tyrosine phosphatase 1B activity in the sperm of obese males impairs the sperm acrosome reaction.The Journal of biological chemistry · 2014Article
- A heterozygous mutation of GALNTL5 affects male infertility with impairment of sperm motility.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Calcium influx and male fertility in the context of the sperm proteome: an update.BioMed research international · 2014Review
- MARCKS protein is phosphorylated and regulates calcium mobilization during human acrosomal exocytosis.PloS one · 2013Article
- Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.The Journal of biological chemistry · 2012Article
- Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- α-SNAP prevents docking of the acrosome during sperm exocytosis because it sequesters monomeric syntaxin.PloS one · 2011Article
- Acrosome reaction: relevance of zona pellucida glycoproteins.Asian journal of andrology · 2011Review
- Calcineurin-mediated dephosphorylation of synaptotagmin VI is necessary for acrosomal exocytosis.The Journal of biological chemistry · 2010Article
- Article
- Sphingosine 1-phosphate and sphingosine kinase are involved in a novel signaling pathway leading to acrosomal exocytosis.The Journal of biological chemistry · 2010Article
- Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis.The Journal of biological chemistry · 2009Article
- PTP1B dephosphorylates N-ethylmaleimide-sensitive factor and elicits SNARE complex disassembly during human sperm exocytosis.The Journal of biological chemistry · 2009Article
- Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules.Journal of experimental botany · 2008Article
- Complexin I is required for mammalian sperm acrosomal exocytosis.Developmental biology · 2007Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The acrosome reaction of spermatozoa is a complex, calcium-dependent, regulated exocytosis. Fusion at multiple sites between the outer acrosomal membrane and the cell membrane causes the release of the acrosomal contents and the loss of the membranes surrounding the acrosome. However, very little is known about the molecules that mediate and regulate this unique fusion process. Here, we show that N-ethylmaleimide-sensitive factor (NSF), a protein essential for most fusion events, is present in the acrosome of several mammalian spermatozoa. Moreover, we demonstrate that calcium-dependent exocytosis of permeabilized sperm requires active NSF. Previously, we have shown that the addition of the active (GTP-bound) form of the small GTPase Rab3A triggers exocytosis in permeabilized spermatozoa. In the present report we show that Rab3A is necessary for calcium-dependent exocytosis. The activation of Rab3A protects NSF from N-ethylmaleimide inhibition and precludes the exchange of the endogenous protein with recombinant dominant negative mutants of NSF. Furthermore, Rab3A activation of acrosomal exocytosis requires active NSF. Our results suggest that, upon calcium stimulation, Rab3A switches to its active GTP-bound form, triggering the formation of a protein complex in which NSF is protected. This process is suggested to be an essential part of the molecular mechanism of membrane fusion leading to the release of the acrosomal contents.
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