ArticleNucleic acids research2000
Differential effect of zinc finger deletions on the binding of CTCF to the promoter of the amyloid precursor protein gene.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 52 citations in OpenAlex.
- Direct analysis of transcription factor protected cfDNA in plasma by ChIP-seq: measurement of altered CTCF binding in cancer is a novel biomarker for liquid biopsy.Clinical epigenetics · 2026Article
- An upstream secondary DNA motif within the IL3 insulator CTCF binding site is required for enhancer-blocking insulator activity.PloS one · 2026Article
- Single-molecule imaging reveals a direct role of CTCF's zinc fingers in SA interaction and cluster-dependent RNA recruitment.Nucleic acids research · 2024Article
- CTCF shapes chromatin structure and gene expression in health and disease.EMBO reports · 2022Review
- Structure-function relationships explain CTCF zinc finger mutation phenotypes in cancer.Cellular and molecular life sciences : CMLS · 2021Article
- Mechanisms of TP53 Pathway Inactivation in Embryonic and Somatic Cells-Relevance for Understanding (Germ Cell) Tumorigenesis.International journal of molecular sciences · 2021Review
- A Newly Assigned Role of CTCF in Cellular Response to Broken DNAs.Biomolecules · 2021Review
- Transcriptional and Post-Transcriptional Regulations of Amyloid-β Precursor ProteinFrontiers in aging · 2021Review
- DNA recognition patterns of the multi-zinc-finger protein CTCF: a mutagenesis study.Acta pharmaceutica Sinica. B · 2018Article
- CTCF-Induced Circular DNA Complexes Observed by Atomic Force Microscopy.Journal of molecular biology · 2018Article
- Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites.Cell research · 2017Article
- Correcting nucleotide-specific biases in high-throughput sequencing data.BMC bioinformatics · 2017Article
- CTCF counter-regulates cardiomyocyte development and maturation programs in the embryonic heart.PLoS genetics · 2017Article
- Active and poised promoter states drive folding of the extended HoxB locus in mouse embryonic stem cells.Nature structural & molecular biology · 2017Article
- Comparative analyses of CTCF and BORIS occupancies uncover two distinct classes of CTCF binding genomic regions.Genome biology · 2015Article
- Emerging landscape of oncogenic signatures across human cancers.Nature genetics · 2013Article
- Characterization of constitutive CTCF/cohesin loci: a possible role in establishing topological domains in mammalian genomes.BMC genomics · 2013Article
- A genome-wide map of CTCF multivalency redefines the CTCF code.Cell reports · 2013Article
- Colocalization of multiple DNA loci: a physical mechanism.Biophysical journal · 2012Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
High levels of transcription from the amyloid precursor protein promoter are dependent on the binding of CTCF to the APBbeta core recognition sequence located between positions -82 and -93 upstream from the transcriptional start site. CTCF comprises 727 amino acids and contains 11 zinc finger motifs arranged in tandem that are flanked by 267 amino acids on the N-terminal side and 150 amino acids on the C-terminal side. Deletion of either the N- or the C-terminal regions outside of the zinc finger domain had no detrimental effect on the binding of CTCF to APBbeta. However, internal deletions of zinc fingers 5-7 completely abolished binding. The binding of full-length CTCF generated a DNase I protected domain extending from position -78 to -116, which was interrupted by a hypersensitive site at position -99. Selective deletions from the N- and C-terminal sides of the zinc finger domain showed that the N-terminal end of the zinc finger domain was aligned toward the transcriptional start site. Furthermore, deletions of zinc fingers peripheral to the essential zinc fingers 5-7 decreased the stability of the binding complex by interrupting sequence-specific interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.