ArticleNucleic acids research2000
Expression of ZmMET1, a gene encoding a DNA methyltransferase from maize, is associated not only with DNA replication in actively proliferating cells, but also with altered DNA methylation status in cold-stressed quiescent cells.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 146 citations in OpenAlex.
- Single-cell chromatin accessibility and cis-regulatory element analyses in plants using the scPlantReg platform.Nature plants · 2026Article
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- Genome-wide DNA methylation and their transgenerational pattern differ in Arabidopsis thaliana populations originated along the elevation of West Himalaya.BMC plant biology · 2024Article
- Recent Advances in Studies of Genomic DNA Methylation and Its Involvement in Regulating Drought Stress Response in Crops.Plants (Basel, Switzerland) · 2024Review
- Role of Epigenetic Factors in Response to Stress and Establishment of Somatic Memory of Stress Exposure in Plants.Plants (Basel, Switzerland) · 2023Review
- Epigenomics in stress tolerance of plants under the climate change.Molecular biology reports · 2023Review
- Genome-wide association analysis provides insights into the genetic basis of photosynthetic responses to low-temperature stress in spring barley.Frontiers in plant science · 2023Article
- Genetic and environmental drivers of large-scale epigenetic variation in Thlaspi arvense.PLoS genetics · 2022Article
- Transgenerationally Transmitted DNA Demethylation of a Spontaneous Epialleles Using CRISPR/dCas9-TET1cd Targeted Epigenetic Editing in Arabidopsis.International journal of molecular sciences · 2022Article
- Multi-Omics Analysis of Low-Temperature Fruiting Highlights the Promising Cultivation Application of the Nutrients Accumulation inJournal of fungi (Basel, Switzerland) · 2022Article
- Epigenetic variation: A major player in facilitating plant fitness under changing environmental conditions.Frontiers in cell and developmental biology · 2022Review
- Moving Beyond DNA Sequence to Improve Plant Stress Responses.Frontiers in genetics · 2022Review
- Genome-wide identification and transcriptional characterization of DNA methyltransferases conferring temperature-sensitive male sterility in wheat.BMC genomics · 2021Article
- Identification of virulence associated milRNAs and their bidirectional targets in Rhizoctonia solani and maize during infection.BMC plant biology · 2021Article
- Evolutionary Dynamics of Transposable Elements Following a Shared Polyploidization Event in the Tribe Andropogoneae.G3 (Bethesda, Md.) · 2020Article
- DNA Methylation Changes Induced by Cold in Psychrophilic and PsychrotolerantMicroorganisms · 2020Article
- Molecular characterization and transcription analysis of DNA methyltransferase genes in tomato (Solanum lycopersicum).Genetics and molecular biology · 2020Article
- Article
- Physiological, epigenetic and genetic regulation in some olive cultivars under salt stress.Scientific reports · 2019Article
- Shared and genetically distinct Zea mays transcriptome responses to ongoing and past low temperature exposure.BMC genomics · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA fragment encoding part of a DNA methyltransferase was isolated from maize. The putative amino acid sequence identically matched that deduced from a genomic sequence in the database (accession no. AF063403), and the corresponding gene was designated as ZmMET1. Bacterially expressed ZmMET1 actively methylated DNA in vitro. Transcripts of ZmMET1 could be shown to exclusively accumulate in actively proliferating cells of the meristems of mesocotyls and root apices, suggesting ZmMET1 expression to be associated with DNA replication. This was confirmed by simultaneous decrease of transcripts of ZmMET1 and histone H3, a marker for DNA replication, in seedlings exposed to wounding, desiccation and salinity, all of which suppress cell division. Cold stress also depressed both transcripts in root tissues. In contrast, however, accumulation of ZmMET1 transcripts in shoot mesocotyls was not affected by cold stress, whereas those for H3 sharply decreased. Such a differential accumulation of ZmMET1 transcripts was consistent with ZmMET1 protein levels as revealed by western blotting. Expression of ZmMET1 is thus coexistent, but not completely dependent on DNA replication. Southern hybridization analysis with a methylation-sensitive restriction enzyme revealed that cold treatment induced demethylation of DNA in the Ac/Ds transposon region, but not in other genes, and that such demethylation primarily occurred in roots. These results suggested that the methylation level was decreased selectively by cold treatment, and that ZmMET1 may, at least partly, prevent such demethylation.
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