ArticleJournal of virology2000
The 17 nucleotides downstream from the env gene stop codon are important for murine leukemia virus packaging.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- NMR Studies of Retroviral Genome Packaging.Viruses · 2020Review
- Review
- Structural dynamics of retroviral genome and the packaging.Frontiers in microbiology · 2011Article
- Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials.Methods in molecular biology (Clifton, N.J.) · 2009Article
- RNA sequences in the Moloney murine leukemia virus genome bound by the Gag precursor protein in the yeast three-hybrid system.Journal of virology · 2004Article
- Viral vector-mediated transduction of a modified thrombospondin-2 cDNA inhibits tumor growth and angiogenesis.Gene therapy · 2004Article
- Multiple modifications allow high-titer production of retroviral vectors carrying heterologous regulatory elements.Journal of virology · 2004Article
- Dasheng and RIRE2. A nonautonomous long terminal repeat element and its putative autonomous partner in the rice genome.Plant physiology · 2002Article
- cis-Acting elements important for retroviral RNA packaging specificity.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified a previously unknown nucleotide sequence important for the packaging of murine leukemia virus. This nucleotide sequence is located downstream from the stop codon of the env gene but does not overlap the polypurine tract. Deletion of 17 bp from this region resulted in a more than 10-fold decrease in viral titer. Consistent with this result, the deletion mutant showed a 20- to 30-fold drop in the amount of virion RNA in the culture supernatant. The total amount of virion protein in the culture supernatant was comparable for the deletion mutant and the parental virus, suggesting that the mutant construct could release the empty viral particles. These results suggested that the packaging signal sequence might be present at the two extreme sites of the viral genome, one in the region around the splice donor sequence downstream from the 5' long terminal repeat (LTR) and the other immediately upstream from the 3' LTR. Implications for gene therapy, especially in regard to construction of retroviral vectors and packaging constructs, are discussed.
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Registered trials
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