ArticleJournal of virology2000
Is the 135S poliovirus particle an intermediate during cell entry?
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Antiviral Development for the Polio Endgame: Current Progress and Future Directions.Pathogens (Basel, Switzerland) · 2024Review
- Enterovirus-D68 - A Reemerging Non-Polio Enterovirus that Causes Severe Respiratory and Neurological Disease in Children.Frontiers in virology (Lausanne, Switzerland) · 2024Article
- Cryo-EM structures reveal two distinct conformational states in a picornavirus cell entry intermediate.PLoS pathogens · 2020Article
- Molecular basis for the acid-initiated uncoating of human enterovirus D68.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Immune Evasion of Enteroviruses Under Innate Immune Monitoring.Frontiers in microbiology · 2018Review
- Antibody-induced uncoating of human rhinovirus B14.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Cryo-electron Microscopy Structures of Expanded Poliovirus with VHHs Sample the Conformational Repertoire of the Expanded State.Journal of virology · 2017Article
- Picornavirus RNA is protected from cleavage by ribonuclease during virion uncoating and transfer across cellular and model membranes.PLoS pathogens · 2017Article
- Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Human enterovirus 71 uncoating captured at atomic resolution.Journal of virology · 2014Article
- Neutralizing antibodies can initiate genome release from human enterovirus 71.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The enterovirus 71 A-particle forms a gateway to allow genome release: a cryoEM study of picornavirus uncoating.PLoS pathogens · 2013Article
- Structure of the Fab-labeled "breathing" state of native poliovirus.Journal of virology · 2012Article
- Insights into minor group rhinovirus uncoating: the X-ray structure of the HRV2 empty capsid.PLoS pathogens · 2012Article
- An externalized polypeptide partitions between two distinct sites on genome-released poliovirus particles.Journal of virology · 2011Article
- Poliovirus RNA is released from the capsid near a twofold symmetry axis.Journal of virology · 2011Article
- Limited trafficking of a neurotropic virus through inefficient retrograde axonal transport and the type I interferon response.PLoS pathogens · 2010Article
- Picornaviruses.Current topics in microbiology and immunology · 2010Article
- Multi-disciplinary studies of viruses: the role of structure in shaping the questions and answers.Journal of structural biology · 2008Review
- Multiple host barriers restrict poliovirus trafficking in mice.PLoS pathogens · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Poliovirus binding to its receptor (PVR) on the cell surface induces a conformational transition which generates an altered particle with a sedimentation value of 135S versus the 160S of the native virion. A number of lines of evidence suggest that the 135S particle is a cell entry intermediate. However, the low infection efficiencies of the 135S particle and the absence of detectable 135S particles during infection at 26 degrees C by the cold-adapted mutants argue against a role for the 135S particle during the cell entry process. We show here that binding of 135S-antibody complexes to the Fc receptor (CDw32) increases the infectivity of these particles by 2 to 3 orders of magnitude. Thus, the low efficiency of infection by 135S particles is due in part to the low binding affinity of these particles. In addition, we show that there is an additional stage in the entry process that is associated with RNA release. This stage occurs after formation of the 135S particle, is rate limiting during infection at 37 degrees C, but not at 26 degrees C, and is PVR independent. The data also demonstrate that during infection at 26 degrees C, the rate-limiting step is the PVR-mediated conversion of wild-type 160S particles to 135S particles. This suggests that during infection at 26 degrees C by the cold-adapted viruses, 135S particles are formed, but they fail to accumulate to detectable levels because the subsequent post-135S particle events occur at a significantly faster rate than the initial conversion of 160S to 135S particles. These data support a model in which the 135S particle is an intermediate during poliovirus entry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.