ArticleJournal of virology2000
Relationship between retroviral DNA integration and gene expression.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Random Insertion Reporter Gimmicks Powered by Cut-and-Paste DNA Transposons.Biomedicines · 2025Review
- Lentivector integration sites in ependymal cells from a model of metachromatic leukodystrophy: non-B DNA as a new factor influencing integration.Molecular therapy. Nucleic acids · 2014Article
- Alpharetroviral self-inactivating vectors: long-term transgene expression in murine hematopoietic cells and low genotoxicity.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas.Journal of virology · 2010Article
- Crystal structures of catalytic core domain of BIV integrase: implications for the interaction between integrase and target DNA.Protein & cell · 2010Article
- Human immunodeficiency virus integration efficiency and site selection in quiescent CD4+ T cells.Journal of virology · 2009Article
- Viral vectors: from virology to transgene expression.British journal of pharmacology · 2009Review
- Integration site selection by retroviral vectors: molecular mechanism and clinical consequences.Human gene therapy · 2008Review
- Viral determinants of integration site preferences of simian immunodeficiency virus-based vectors.Journal of virology · 2006Article
- Simian immunodeficiency virus integration preference is similar to that of human immunodeficiency virus type 1.Journal of virology · 2005Article
- Integration targeting by avian sarcoma-leukosis virus and human immunodeficiency virus in the chicken genome.Journal of virology · 2005Article
- Matrix attachment regions as targets for retroviral integration.Virology journal · 2005Article
- High-resolution genome-wide mapping of transposon integration in mammals.Molecular and cellular biology · 2005Article
- Relationship between retroviral DNA-integration-site selection and host cell transcription.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The ups and downs of gene expression and retroviral DNA integration.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Vector integration: location, location, location.Gene therapy · 2005Article
- Genome-wide analyses of avian sarcoma virus integration sites.Journal of virology · 2004Article
- Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences.PLoS biology · 2004Article
- HIV-1 gene expression: lessons from provirus and non-integrated DNA.Retrovirology · 2004Review
- Integrase-specific enhancement and suppression of retroviral DNA integration by compacted chromatin structure in vitro.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although retroviruses can integrate their DNA into a large number of sites in the host genome, factors controlling the specificity of integration remain controversial and poorly understood. To assess the effects of transcriptional activity on integration in vivo, we created quail cell clones containing a construct with a minigene cassette, whose expression is controlled by the papilloma virus E2 protein. From these clones we derived transcriptionally active subclones expressing the wild-type E2 protein and transcriptionally silent subclones expressing a mutant E2 protein that binds its target DNA but is unable to activate transcription. By infecting both clones and subclones with avian leukosis virus and using a PCR-based assay to determine viral DNA integration patterns, we were able to assess the effects of both protein binding and transcriptional activity on retroviral DNA integration. Contrary to the hypothesis that transcriptional activity enhances integration, we found an overall decrease in integration into our gene cassette in subclones expressing the wild-type E2 protein. We also found a decrease in integration into our gene cassette in subclones expressing the mutant E2 protein, but only into the protein binding region. Based on these findings, we propose that transcriptionally active DNA is not a preferred target for retroviral integration and that transcriptional activity may in fact be correlated with a decrease in integration.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.