ArticleThe Journal of clinical investigation2000
Thrombin activates a Y box-binding protein (DNA-binding protein B) in endothelial cells.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- YBX1/PPIB axis promotes post-maturation arteriovenous fistula stenosis via enhancing endothelial to mesenchymal transition.Scientific reports · 2026Article
- YB1 dephosphorylation attenuates atherosclerosis by promoting CCL2 mRNA decay.Frontiers in cardiovascular medicine · 2022Article
- Review
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- Cold shock proteins: from cellular mechanisms to pathophysiology and disease.Cell communication and signaling : CCS · 2018Review
- RSK-mediated nuclear accumulation of the cold-shock Y-box protein-1 controls proliferation of T cells and T-ALL blasts.Cell death and differentiation · 2017Article
- Article
- Activated Protein C Ameliorates Renal Ischemia-Reperfusion Injury by Restricting Y-Box Binding Protein-1 Ubiquitination.Journal of the American Society of Nephrology : JASN · 2015Article
- The role of cold shock domain proteins in inflammatory diseases.Journal of molecular medicine (Berlin, Germany) · 2014Review
- Article
- The regulation of AβPP expression by RNA-binding proteins.Ageing research reviews · 2012Review
- Thrombin induces endothelial arginase through AP-1 activation.American journal of physiology. Cell physiology · 2010Article
- Genotoxic stress-induced nuclear localization of oncoprotein YB-1 in the absence of proteolytic processing.Oncogene · 2010Article
- Article
- Article
- Proteasome-mediated cleavage of the Y-box-binding protein 1 is linked to DNA-damage stress response.The EMBO journal · 2005Article
- YB-1 coordinates vascular smooth muscle alpha-actin gene activation by transforming growth factor beta1 and thrombin during differentiation of human pulmonary myofibroblasts.Molecular biology of the cell · 2005Article
- Article
- YB-1 is important for late-stage embryonic development, optimal cellular stress responses, and the prevention of premature senescence.Molecular and cellular biology · 2005Article
- Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling.The EMBO journal · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Thrombin stimulates the expression of multiple genes in endothelial cells (ECs), but the trans-acting factors responsible for this induction remain undefined. We have previously described a thrombin-inducible nuclear factor (TINF), which binds to an element in the PDGF B promoter and is responsible for the thrombin inducibility of this gene. Inactive cytoplasmic TINF is rapidly activated and translocated to nuclei of ECs upon stimulation with thrombin. We have now purified TINF from thrombin-treated ECs. Amino acid sequencing revealed it to be a member of the Y-box protein family, and the sole Y-box protein-encoding cDNA we detected in human or bovine ECs corresponded to DNA-binding protein B (dbpB). DbpB translocated to the nucleus after thrombin stimulation of ECs as shown by FACS analysis of nuclei from ECs expressing GFP-dbpB fusion proteins. During thrombin activation, dbpB was found to be cleaved, yielding a 30-kDa NH(2)-terminal fragment that recognized the thrombin-response element sequence, but not the Y-box consensus sequence. Preincubation of ECs with protein tyrosine phosphatase inhibitors completely blocked dbpB activation by thrombin and blocked induction of endogenous PDGF B-chain mRNA and promoter activation by thrombin. Y-box proteins are known to act constitutively to regulate the expression of several genes. Activation of this class of transcription factors in response to thrombin or any other agonist represents a novel signaling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.