ArticleThe Journal of cell biology2000
Differential regulation of granule-to-granule and granule-to-plasma membrane fusion during secretion from rat pituitary lactotrophs.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 81 citations in OpenAlex.
- Resistance exercise stress: theoretical mechanisms for growth hormone processing and release from the anterior pituitary somatotroph.European journal of applied physiology · 2023Review
- CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes.Nature communications · 2022Article
- Effects of Excess Brain-Derived Human α-Synuclein on Synaptic Vesicle Trafficking.Frontiers in neuroscience · 2021Article
- Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action.Scientific reports · 2019Article
- Anaphylactic Degranulation of Mast Cells: Focus on Compound Exocytosis.Journal of immunology research · 2019Review
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis.Scientific reports · 2017Article
- Spatiotemporal detection and analysis of exocytosis reveal fusion "hotspots" organized by the cytoskeleton in endocrine cells.Biophysical journal · 2015Article
- Differential cargo mobilisation within Weibel-Palade bodies after transient fusion with the plasma membrane.PloS one · 2014Article
- A new role for myosin II in vesicle fission.PloS one · 2014Article
- cAMP-mediated stabilization of fusion pores in cultured rat pituitary lactotrophs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Dependence of the excitability of pituitary cells on cyclic nucleotides.Journal of neuroendocrinology · 2012Review
- Multivesicular exocytosis in rat pancreatic beta cells.Diabetologia · 2012Article
- Regulation of fusion pore closure and compound exocytosis in neuroendocrine PC12 cells by SCAMP1.Traffic (Copenhagen, Denmark) · 2011Article
- Ion channels and signaling in the pituitary gland.Endocrine reviews · 2010Review
- Inhibitors of the V0 subunit of the vacuolar H+-ATPase prevent segregation of lysosomal- and secretory-pathway proteins.Journal of cell science · 2009Article
- Trafficking and fusion of neuropeptide Y-containing dense-core granules in astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Lateral drift correction in time-laps images by the particle-tracking algorithm.European biophysics journal : EBJ · 2008Article
- The color of lactotroph secretory granules stained with FM1-43 depends on dye concentration.Biophysical journal · 2008Article
- Subnanometer fusion pores in spontaneous exocytosis of peptidergic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used fluorescence imaging of individual exocytic events together with electron microscopy to study the regulation of dense core granule-to-plasma membrane fusion and granule-to-granule fusion events that occur during secretion from rat pituitary lactotrophs. Stimulating secretion with elevated extracellular potassium, with the calcium ionophore ionomycin, or with thyrotropin releasing hormone or vasoactive intestinal polypeptide resulted in abundant exocytic structures. Approximately 67% of these structures consisted of multiple granules fused together sharing a single exocytic opening with the plasma membrane, i.e., compound exocytosis. For all of these stimulation conditions there appeared to be a finite number of plasma membrane fusion sites, approximately 11 sites around each cellular equator. However, a granule could fuse directly with another granule that had already fused with the plasma membrane even before all plasma membrane sites were occupied. Granule-to-plasma membrane and granule-to-granule fusion events were subject to different regulations. Forskolin, which can elevate cAMP, increased the number of granule-to-granule fusion events without altering the number of granule-to-plasma membrane fusion events. In contrast, the phorbol ester PMA, which activates protein kinase C increased both granule-to-granule and granule-to-plasma membrane fusion events. These results provide a cellular mechanism that can account for the previously demonstrated potentiation of secretion from lactotrophs by cAMP- and PKC-dependent pathways.
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