ArticleThe Journal of cell biology2000
Two pathways through Cdc42 couple the N-formyl receptor to actin nucleation in permeabilized human neutrophils.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 109 citations in OpenAlex.
- The Histamine Four Receptor Regulates LFA-1 But Not Mac-1 -Dependent Neutrophil Adhesion and Inflammatory Functions.Inflammation · 2026Article
- Article
- Targeting Endothelial Barrier Dysfunction Caused by Circulating Bacterial and Mitochondrial N-Formyl Peptides With Deformylase.Frontiers in immunology · 2019Article
- The role of the LTBSeminars in immunology · 2017Review
- Altering the threshold of an excitable signal transduction network changes cell migratory modes.Nature cell biology · 2017Article
- Locally excitable Cdc42 signals steer cells during chemotaxis.Nature cell biology · 2016Article
- Formyl Peptide Receptor Activation Elicits Endothelial Cell Contraction and Vascular Leakage.Frontiers in immunology · 2016Article
- Neutrophils generate microparticles during exposure to inert gases due to cytoskeletal oxidative stress.The Journal of biological chemistry · 2014Article
- RNA therapeutics targeting osteoclast-mediated excessive bone resorption.Advanced drug delivery reviews · 2012Review
- The Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration.The Journal of cell biology · 2012Article
- Neutrophil beta2 integrin inhibition by enhanced interactions of vasodilator-stimulated phosphoprotein with S-nitrosylated actin.The Journal of biological chemistry · 2011Article
- Identification of phosphoproteins associated with human neutrophil granules following chemotactic peptide stimulation.Molecular & cellular proteomics : MCP · 2011Article
- Myristoylated alanine-rich C-kinase substrate (MARCKS) protein regulation of human neutrophil migration.American journal of respiratory cell and molecular biology · 2010Article
- Cdc42 regulates Fc gamma receptor-mediated phagocytosis through the activation and phosphorylation of Wiskott-Aldrich syndrome protein (WASP) and neural-WASP.Molecular biology of the cell · 2009Article
- Adaptor protein SLAT modulates Fcgamma receptor-mediated phagocytosis in murine macrophages.The Journal of biological chemistry · 2009Article
- A common cofilin activity cycle in invasive tumor cells and inflammatory cells.Journal of cell science · 2009Article
- Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodeling.American journal of physiology. Cell physiology · 2008Article
- Rac1 and Rac2 differentially regulate actin free barbed end formation downstream of the fMLP receptor.The Journal of cell biology · 2007Article
- Rac1 links leading edge and uropod events through Rho and myosin activation during chemotaxis.Blood · 2006Article
- Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis.Blood · 2006Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We developed a permeabilization method that retains coupling between N-formyl-methionyl-leucyl-phenylalanine tripeptide (FMLP) receptor stimulation, shape changes, and barbed-end actin nucleation in human neutrophils. Using GTP analogues, phosphoinositides, a phosphoinositide-binding peptide, constitutively active or inactive Rho GTPase mutants, and activating or inhibitory peptides derived from neural Wiskott-Aldrich syndrome family proteins (N-WASP), we identified signaling pathways leading from the FMLP receptor to actin nucleation that require Cdc42, but then diverge. One branch traverses the actin nucleation pathway involving N-WASP and the Arp2/3 complex, whereas the other operates through active Rac to promote actin nucleation. Both pathways depend on phosphoinositide expression. Since maximal inhibition of the Arp2/3 pathway leaves an N17Rac inhibitable alternate pathway intact, we conclude that this alternate involves phosphoinositide-mediated uncapping of actin filament barbed ends.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.