ArticleBritish journal of pharmacology2000
Sphingosine-1-phosphate and sphingosylphosphorylcholine constrict renal and mesenteric microvessels in vitro.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 103 citations in OpenAlex.
- Sphingosine-1-Phosphate Receptor 2 Promotes Renal Microvascular Constriction and Kidney Injury Following Renal Ischemia-Reperfusion in Rats.Function (Oxford, England) · 2025Article
- Sphingolipids and Chronic Kidney Disease.Journal of clinical medicine · 2024Review
- Inhibition of S1P Receptor 2 Attenuates Endothelial Dysfunction and Inhibits Atherogenesis in Apolipoprotein E-Deficient Mice.Journal of atherosclerosis and thrombosis · 2021Article
- Sphingosine-1-Phosphate Enhances αInternational journal of molecular sciences · 2019Article
- The Functional Requirement for CD69 in Establishment of Resident Memory CD8Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- cAMP guided his way: a life for G protein-mediated signal transduction and molecular pharmacology-tribute to Karl H. Jakobs.Naunyn-Schmiedeberg's archives of pharmacology · 2019Review
- Requirement for sphingosine kinase 1 in mediating phase 1 of the hypotensive response to anandamide in the anaesthetised mouse.European journal of pharmacology · 2019Article
- Dietary Fatty Acid Saturation Modulates Sphingosine-1-Phosphate-Mediated Vascular Function.Journal of diabetes research · 2019Article
- Mechanisms of sphingosine-1-phosphate-mediated vasoconstriction of rat afferent arterioles.Acta physiologica (Oxford, England) · 2018Article
- S1P Signaling and De Novo Biosynthesis in Blood Pressure Homeostasis.The Journal of pharmacology and experimental therapeutics · 2016Review
- Emerging biology of sphingosine-1-phosphate: its role in pathogenesis and therapy.The Journal of clinical investigation · 2015Review
- Sphingosine-1-phosphate evokes unique segment-specific vasoconstriction of the renal microvasculature.Journal of the American Society of Nephrology : JASN · 2014Article
- Short-term Mg deficiency upregulates protein kinase C isoforms in cardiovascular tissues and cells; relation to NF-kB, cytokines, ceramide salvage sphingolipid pathway and PKC-zeta: hypothesis and review.International journal of clinical and experimental medicine · 2014Article
- Sphingosine-1-phosphate induced contraction of bladder smooth muscle.European journal of pharmacology · 2013Article
- Article
- Signaling pathway of lysophosphatidic Acid-induced contraction in feline esophageal smooth muscle cells.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2013Article
- Effects of sphingosine-1-phosphate on pacemaker activity of interstitial cells of Cajal from mouse small intestine.Molecules and cells · 2013Article
- Emerging role of G protein-coupled receptors in microvascular myogenic tone.Cardiovascular research · 2012Review
- Role of sphingosine-1-phosphate in the renal medulla.American journal of physiology. Renal physiology · 2011Article
- Short-term magnesium deficiency upregulates sphingomyelin synthase and p53 in cardiovascular tissues and cells: relevance to the de novo synthesis of ceramide.American journal of physiology. Heart and circulatory physiology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sphingolipids such as sphingosine-1-phosphate (SPP) and sphingosylphosphorylcholine (SPPC) can act both intracellularly and at G-protein-coupled receptors, some of which were cloned and designated as Edg-receptors. Sphingolipid-induced vascular effects were determined in isolated rat mesenteric and intrarenal microvessels. Additionally, sphingolipid-induced elevations in intracellular Ca(2+) concentration were measured in cultured rat aortic smooth muscle cells. SPPC and SPP (0.1-100 micromol l(-1)) caused concentration-dependent contraction of mesenteric and intrarenal microvessels (e.g. SPPC in mesenteric microvessels pEC(50) 5.63+/-0.17 and E(max) 49+/-3% of noradrenaline), with other sphingolipids being less active. The vasoconstrictor effect of SPPC in mesenteric microvessels was stereospecific (pEC(50) D-erythro-SPPC 5.69+/-0.08, L-threo-SPPC 5.31+/-0.06) and inhibited by pretreatment with pertussis toxin (E(max) from 44+/-5 to 19+/-4%), by chelation of extracellular Ca(2+) with EGTA and by nitrendipine (E(max) from 40+/-6 to 6+/-1 and 29+/-6%, respectively). Mechanical endothelial denudation or NO synthase inhibition did not alter the SPPC effects, while indomethacin reduced them (E(max) from 87+/-3 to 70+/-4%). SPP and SPPC caused transient increases in intracellular Ca(2+) concentrations in rat aortic smooth muscle cells in a pertussis toxin-sensitive manner. Our data demonstrate that SPP and SPPC cause vasoconstriction of isolated rat microvessels and increase intracellular Ca(2+) concentrations in cultured rat aortic smooth muscle cells. These effects appear to occur via receptors coupled to pertussis toxin-sensitive G-proteins. This is the first demonstration of effects of SPP and SPPC on vascular tone and suggests that sphingolipids may be an hitherto unrecognized class of endogenous regulators of vascular tone.
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