ArticleBritish journal of pharmacology2000
Ligand regulation of green fluorescent protein-tagged forms of the human beta(1)- and beta(2)-adrenoceptors; comparisons with the unmodified receptors.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 76 citations in OpenAlex.
- Light-Based Molecular Tools to Precisely Monitor and Operate β-Adrenoceptors.Medicinal research reviews · 2026Review
- Adrenoceptor Desensitization: Current Understanding of Mechanisms.Pharmacological reviews · 2024Review
- Asthma and COPD: A Focus on β-Agonists - Past, Present and Future.Handbook of experimental pharmacology · 2024Review
- Knock-In Mouse Models to Investigate the Functions of Opioid ReceptorsFrontiers in cellular neuroscience · 2022Review
- The Delta-Opioid Receptor; a Target for the Treatment of Pain.Frontiers in molecular neuroscience · 2020Review
- Molecular Pharmacology of δ-Opioid Receptors.Pharmacological reviews · 2016Review
- Recent advances on the δ opioid receptor: from trafficking to function.British journal of pharmacology · 2015Review
- Fluorescent knock-in mice to decipher the physiopathological role of G protein-coupled receptors.Frontiers in pharmacology · 2014Review
- A novel approach to quantify G-protein-coupled receptor dimerization equilibrium using bioluminescence resonance energy transfer.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Activation of spinal mu- and delta-opioid receptors potently inhibits substance P release induced by peripheral noxious stimuli.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Cardiomyocyte lipids impair β-adrenergic receptor function via PKC activation.American journal of physiology. Endocrinology and metabolism · 2011Article
- Human beta1-adrenergic receptor is subject to constitutive and regulated N-terminal cleavage.The Journal of biological chemistry · 2010Article
- Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Effects of inactivation-resistant agonists on the signalling, desensitization and down-regulation of bradykinin B(2) receptors.British journal of pharmacology · 2009Article
- Mdm2 directs the ubiquitination of beta-arrestin-sequestered cAMP phosphodiesterase-4D5.The Journal of biological chemistry · 2009Article
- Rapid recycling of beta-adrenergic receptors is dependent on the actin cytoskeleton and myosin Vb.Traffic (Copenhagen, Denmark) · 2008Article
- Importance of a N-terminal aspartate in the internalization of the neuropeptide Y Y2 receptor.European journal of pharmacology · 2008Article
- Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes.Nature biotechnology · 2007Article
- Validation of a rapid, non-radioactive method to quantify internalisation of G-protein coupled receptors.Naunyn-Schmiedeberg's archives of pharmacology · 2007Article
- Anti-beta1-adrenergic receptor autoantibodies in patients with chronic Chagas heart disease.Clinical and experimental immunology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable clones of HEK293 cells expressing either FLAG(TM) epitope-tagged, wild type human beta(1)- and beta(2)-adrenoceptors or C-terminally green fluorescent protein (GFP)-tagged forms of these receptors were established. The binding affinity of [(3)H]-dihydroalprenolol and other ligands was little affected by addition of GFP to the C-terminal of either receptor. Isoprenaline induced the internalisation of both beta(1)-adrenoceptor-GFP and beta(2)-adrenoceptor-GFP and following removal of the agonist both constructs were able to recycle to the cell surface. The extent of internalisation of beta(2)-adrenoceptor-GFP produced by isoprenaline was substantially greater than for beta(1)-adrenoceptor-GFP. C-terminal addition of GFP slowed markedly the rate of internalization of both the beta(1)-adrenoceptor and the beta(2)-adrenoceptor in response to isoprenaline. Sustained exposure to isoprenaline (24 h) produced substantially greater levels of downregulation of native beta(2)-adrenoceptor compared to beta(2)-adrenoceptor-GFP although both were equally effectively removed from the plasma membrane. Sustained exposure to isoprenaline resulted in a large fraction of beta(2)-adrenoceptor-GFP becoming trapped in internal vesicles/lysosomes but not degraded. Even after sustained exposure to isoprenaline a significant fraction of beta(1)-adrenoceptor-GFP remained at the cell surface. These results indicate that although GFP tagging of beta-adrenoceptors can provide qualitative visual patterns of agonist-induced receptor trafficking and regulation in HEK293 cells the quantitative details vary markedly from those obtained with the unmodified receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.