ArticleBritish journal of pharmacology2000
Calcium-pH crosstalks in rat mast cells: cytosolic alkalinization, but not intracellular calcium release, is a sufficient signal for degranulation.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Sub-Second Ratiometric Fluorescence Imaging of Histamine Dynamics in Living Cells via a Supramolecular Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cross talk between polysulfide and nitric oxide in rat peritoneal mast cells.American journal of physiology. Cell physiology · 2016Article
- Simultaneous pH measurement in endocytic and cytosolic compartments in living cells using confocal microscopy.Journal of visualized experiments : JoVE · 2014Article
- Mast cell mediators: their differential release and the secretory pathways involved.Frontiers in immunology · 2014Review
- Prostaglandin E2 prevents hyperosmolar-induced human mast cell activation through prostanoid receptors EP2 and EP4.PloS one · 2014Article
- Intracellular alkalinization induces cytosolic Ca2+ increases by inhibiting sarco/endoplasmic reticulum Ca2+-ATPase (SERCA).PloS one · 2012Article
- Salinomycin inhibits Wnt signaling and selectively induces apoptosis in chronic lymphocytic leukemia cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Induction of intracellular Ca2+ and pH changes in Sf9 insect cells by rhodojaponin-III, a natural botanic insecticide isolated from Rhododendron molle.Molecules (Basel, Switzerland) · 2011Article
- Intracellular pH modulates inner segment calcium homeostasis in vertebrate photoreceptors.American journal of physiology. Cell physiology · 2011Article
- P2Y(13) receptor is responsible for ADP-mediated degranulation in RBL-2H3 rat mast cells.Pharmacological research · 2010Article
- Effects of 4-aminopyridine on organelle movement in cultured mouse dorsal root ganglion neurites.Journal of molecular neuroscience : MN · 2010Article
- Ragweed pollen-mediated IgE-independent release of biogenic amines from mast cells via induction of mitochondrial dysfunction.Molecular immunology · 2009Article
- Intracellular alkalization causes pain sensation through activation of TRPA1 in mice.The Journal of clinical investigation · 2008Article
- Ca2+ signaling, intracellular pH and cell volume in cell proliferation.The Journal of membrane biology · 2005Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this work was to study the relationship between intracellular alkalinization, calcium fluxes and histamine release in rat mast cells. Intracellular alkalinization was induced by nigericin, a monovalent cation ionophore, and by NH(4)Cl (ammonium chloride). Calcium cytosolic and intracellular pH were measured by fluorescence digital imaging using Fura-2-AM and BCECF-AM. In rat mast cells, nigericin and NH(4)Cl induce a dose-dependent intracellular alkalinization, a dose-dependent increase in intracellular calcium levels by releasing calcium from intracellular pools, and an activation of capacitative calcium influx. The increase in both intracellular calcium and pH activates exocytosis (histamine release) in the absence of external calcium. Under the same conditions, thapsigargin does not activate exocytosis, the main difference being that thapsigargin does not alkalinize the cytosol. After alkalinization, histamine release is intracellular-calcium dependent. With 2.5 mM EGTA and thapsigargin the cell response decreases by 62%. The cytosolic alkalinization, in addition to the calcium increase it is enough signal to elicit the exocytotic process in rat mast cells.
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Registered trials
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