ArticleAntimicrobial agents and chemotherapy2000
Molecular characterization of the plasma membrane H(+)-ATPase, an antifungal target in Cryptococcus neoformans.
Article in Antimicrobial agents and chemotherapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Antimicrobial Coatings for Medical Textiles via Reactive Organo-Selenium Compounds.Molecules (Basel, Switzerland) · 2023Article
- Article
- Ergosterol distribution controls surface structure formation and fungal pathogenicity.bioRxiv : the preprint server for biology · 2023Article
- In vitro antifungal activity of dimethyl trisulfide against Colletotrichum gloeosporioides from mango.World journal of microbiology & biotechnology · 2019Article
- Article
- Promising Antifungal Targets AgainstFrontiers in cellular and infection microbiology · 2018Review
- The Celecoxib Derivative AR-12 Has Broad-Spectrum Antifungal Activity In Vitro and Improves the Activity of Fluconazole in a Murine Model of Cryptococcosis.Antimicrobial agents and chemotherapy · 2016Article
- Antifungal Mechanism of Action of Lactoferrin: Identification of H+-ATPase (P3A-Type) as a New Apoptotic-Cell Membrane Receptor.Antimicrobial agents and chemotherapy · 2016Article
- The Mechanistic Targets of Antifungal Agents: An Overview.Mini reviews in medicinal chemistry · 2016Review
- Demethoxycurcumin Is A Potent Inhibitor of P-Type ATPases from Diverse Kingdoms of Life.PloS one · 2016Article
- Inositol phosphosphingolipid phospholipase C1 regulates plasma membrane ATPase (Pma1) stability in Cryptococcus neoformans.FEBS letters · 2014Article
- Advances in targeting the vacuolar proton-translocating ATPase (V-ATPase) for anti-fungal therapy.Frontiers in pharmacology · 2014Review
- Regulation of Saccharomyces cerevisiae Plasma membrane H(+)-ATPase (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress.Indian journal of microbiology · 2011Article
- Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A.BMC microbiology · 2011Article
- Ebselen is a potent non-competitive inhibitor of extracellular nucleoside diphosphokinase.Purinergic signalling · 2010Article
- Cryptococcal interactions with the host immune system.Eukaryotic cell · 2010Review
- A putative P-type ATPase, Apt1, is involved in stress tolerance and virulence in Cryptococcus neoformans.Eukaryotic cell · 2010Article
- Mathematical modeling of pathogenicity of Cryptococcus neoformans.Molecular systems biology · 2008Article
- The cryptococcal enzyme inositol phosphosphingolipid-phospholipase C confers resistance to the antifungal effects of macrophages and promotes fungal dissemination to the central nervous system.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cryptococcus neoformans PMA1 gene, encoding a plasma membrane H(+)-ATPase, was isolated from a genomic DNA library of serotype A strain ATCC 6352. An open reading frame of 3,380 nucleotides contains six introns and encodes a predicted protein consisting of 998 amino acids with a molecular mass of approximately 108 kDa. Plasma membranes were isolated, and the H(+)-ATPase was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be slightly larger than the S. cerevisiae H(+)-ATPase, consistent with its predicted molecular mass. The plasma membrane-bound enzyme exhibited a pH 6.5 optimum for ATP hydrolysis, K(m) and V(max) values of 0.5 mM and 3.1 micromol mg(-1) min(-1), respectively, and an apparent K(i) for vanadate inhibition of 1.6 microM. ATP hydrolysis in plasma membranes and medium acidification by whole cells were inhibited by ebselen, a nonspecific H(+)-ATPase antagonist which was also fungicidal. The predicted C. neoformans protein is 35% identical to proton pumps of both pathogenic and nonpathogenic fungi but exhibits more than 50% identity to PMA1 genes from plants. Collectively, this study provides the basis for establishing the Cryptococcus H(+)-ATPase as a viable target for antifungal drug discovery.
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