ArticleOncogene2000
Immuno-histochemical detection of human telomerase reverse transcriptase in human liver tissues.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 55 citations in OpenAlex.
- Review
- HCV core inhibits hepatocellular carcinoma cell replicative senescence through downregulating microRNA-138 expression.Journal of molecular medicine (Berlin, Germany) · 2017Article
- Treatment of Liver Cancer.Cold Spring Harbor perspectives in medicine · 2015Review
- Current systemic treatment of hepatocellular carcinoma: A review of the literature.World journal of hepatology · 2015Review
- Stem cell therapy for inherited metabolic disorders of the liver.Experimental hematology · 2008Review
- Nuclear telomerase is less accessible to antibody probing than known nuclear antigens: retrieval with new immunostaining buffer.Histochemistry and cell biology · 2005Article
- Expression of human telomerase reverse transcriptase in thyroid follicular neoplasms: an immunohistochemical study.Endocrine pathology · 2005Article
- Differential expression of telomerase reverse transcriptase (hTERT) in lung tumours.British journal of cancer · 2004Article
- Significance of immunological detection of human telomerase reverse transcriptase: re-evaluation of expression and localization of human telomerase reverse transcriptase.The American journal of pathology · 2003Article
- Telomerase-dependent oncolytic adenovirus for cancer treatment.Gene therapy · 2003Article
- Article
- Telomerase inhibition and telomere loss in BEL-7404 human hepatoma cells treated with doxorubicin.World journal of gastroenterology · 2002Article
- Expression profile of a gamma-deletion variant of the human telomerase reverse transcriptase gene.Neoplasia (New York, N.Y.)Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although telomerase activity in hepatocellular carcinoma (HCC) increases in accordance with degree of histological undifferentiation, it is unknown whether the level of telomerase activity in HCC reflects of the degree of activity in individual cells or the frequency of telomerase-positive HCC cells. Non-cancerous liver tissues exhibit low but significant levels of telomerase activity, but the nature of telomerase-positive cells in these tissues is unclear. In this study, we performed immunohistochemical staining using specific antibody against telomerase reverse transcriptase (hTERT) protein in 15 HCC samples and 13 adjacent non-cancerous liver tissues. There were hTERT-positive hepatocytes, though very low frequency, in non-cancerous liver tissues. The frequencies in hTERT positive hepatocytes were very well correlated with clinicopathological parameters and telomerase activity levels: the average frequencies of chronic hepatitis was 0.2%, liver cirrhosis 0.2%, well-differentiated HCC 3.0%, moderately differentiated HCC 28%, and poorly differentiated HCC 95%. The intensity of staining varied among cells within a given specimen, and correlation with degree of histological undifferentiation was less obvious. Portions of migrating lymphocytes and biliary epithelial cells were also hTERT-positive. These findings indicate that the upregulation of telomerase activity with degree of undifferentiation of HCC is mainly due to the increase in frequency of hTERT positive HCC cells.
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