ArticleGenes & development2000
Chromatin fine structure profiles for a developmentally regulated gene: reorganization of the lysozyme locus before trans-activator binding and gene expression.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 92 citations in OpenAlex.
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- Exogenous maltose enhances Zebrafish immunity to levofloxacin-resistant Vibrio alginolyticus.Microbial biotechnology · 2020Article
- The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus.Scientific reports · 2018Article
- Hierarchies of NF-κB target-gene regulation.Nature immunology · 2011Review
- Transcription of ncDNA: Many roads lead to local gene regulation.Transcription · 2011Article
- cis-Regulatory remodeling of the SCL locus during vertebrate evolution.Molecular and cellular biology · 2010Article
- Pioneer factors in embryonic stem cells and differentiation.Current opinion in genetics & development · 2010Review
- Derepression of Polycomb targets during pancreatic organogenesis allows insulin-producing beta-cells to adopt a neural gene activity program.Genome research · 2010Article
- A conserved insulator that recruits CTCF and cohesin exists between the closely related but divergently regulated interleukin-3 and granulocyte-macrophage colony-stimulating factor genes.Molecular and cellular biology · 2009Article
- The LPS-induced transcriptional upregulation of the chicken lysozyme locus involves CTCF eviction and noncoding RNA transcription.Molecular cell · 2008Article
- TSA downregulates Wilms tumor gene 1 (Wt1) expression at multiple levels.Nucleic acids research · 2008Article
- The role of the chromatin remodeler Mi-2beta in hematopoietic stem cell self-renewal and multilineage differentiation.Genes & development · 2008Article
- C/EBPbeta induces chromatin opening at a cell-type-specific enhancer.Molecular and cellular biology · 2008Article
- PAP-LMPCR for improved, allele-specific footprinting and automated chromatin fine structure analysis.Nucleic acids research · 2008Article
- A two-step, PU.1-dependent mechanism for developmentally regulated chromatin remodeling and transcription of the c-fms gene.Molecular and cellular biology · 2007Article
- Oncogenic point mutations in the Myb DNA-binding domain alter the DNA-binding properties of Myb at a physiological target gene.Nucleic acids research · 2007Article
- The mechanism of repression of the myeloid-specific c-fms gene by Pax5 during B lineage restriction.The EMBO journal · 2006Article
- Developmental activation of the lysozyme gene in chicken macrophage cells is linked to core histone acetylation at its enhancer elements.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The chicken lysozyme locus is activated in a stepwise fashion during myeloid differentiation. We have used this locus as a model to study at high resolution changes in chromatin structure both in chicken cell lines representing various stages of macrophage differentiation and in primary cells from transgenic mice. In this study we have addressed the question of whether chromatin rearrangements can be detected in myeloid precursor cells at a stage well before overt transcription of the lysozyme gene begins. In addition to restriction enzyme accessibility assays and DMS footprinting, we have applied new, very sensitive techniques to assay for chromatin changes. Particularly informative was UV photofootprinting, using terminal transferase-dependent PCR and nonradioactive detection. We find that the basic chromatin structure in lysozyme nonexpressing hematopoietic precursor cells is highly similar to the pattern found in fully differentiated lysozyme-expressing cells. In addition, we find that only in nonexpressing cells are dimethylsulfate footprints and UV photofootprints affected by trichostatin, an inhibitor of histone deacetylation. These results are interpreted to mean that most chromatin pattern formation is complete before the binding of end-stage trans-activators, supporting the notion that heritable chromatin structure is central to the stable epigenetic programs that guide development.
Indexed as
Identifiers
10950873PMC316862W2128878636What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.