ArticleThe Plant cell2000
Identification of a calmodulin-regulated soybean Ca(2+)-ATPase (SCA1) that is located in the plasma membrane.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 108 citations in OpenAlex.
- OsACA9, an Autoinhibited CaInternational journal of molecular sciences · 2024Article
- Subcellular Proteomics to Elucidate Soybean Response to Abiotic Stress.Plants (Basel, Switzerland) · 2023Review
- Transcriptome changes induced by Arbuscular mycorrhizal symbiosis in leaves of durum wheat (Triticum durum Desf.) promote higher salt tolerance.Scientific reports · 2023Article
- The calcium connection: exploring the intricacies of calcium signaling in plant-microbe interactions.Frontiers in plant science · 2023Review
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Evolutionary and Regulatory Pattern Analysis of Soybean CaFrontiers in plant science · 2022Article
- Fucoidan treatment alleviates chilling injury in cucumber by regulating ROS homeostasis and energy metabolism.Frontiers in plant science · 2022Article
- Calcium channels and transporters: Roles in response to biotic and abiotic stresses.Frontiers in plant science · 2022Review
- Genome-Wide Analysis of the Soybean Calmodulin-Binding Protein 60 Family and Identification of GmCBP60A-1 Responses to Drought and Salt Stresses.International journal of molecular sciences · 2021Article
- StCaM2, a calcium binding protein, alleviates negative effects of salinity and drought stress in tobacco.Plant molecular biology · 2021Article
- Cellular Localization of Exogenous Cry1Ab/c and its Interaction with Plasma Membrane CaFrontiers in bioengineering and biotechnology · 2021Article
- Article
- The SoybeanInternational journal of molecular sciences · 2020Article
- Transcriptome response of roots to salt stress in a salinity-tolerant bread wheat cultivar.PloS one · 2019Article
- Tissue-specific root ion profiling reveals essential roles of the CAX and ACA calcium transport systems in response to hypoxia in Arabidopsis.Journal of experimental botany · 2016Article
- Functional characterization of a Glycine soja Ca(2+)ATPase in salt-alkaline stress responses.Plant molecular biology · 2016Article
- Soybean Salt Tolerance 1 (GmST1) Reduces ROS Production, Enhances ABA Sensitivity, and Abiotic Stress Tolerance in Arabidopsis thaliana.Frontiers in plant science · 2016Article
- Involvement of calmodulin and calmodulin-like proteins in plant responses to abiotic stresses.Frontiers in plant science · 2015Review
- Abiotic stress responses in plants: roles of calmodulin-regulated proteins.Frontiers in plant science · 2015Review
- ACA12 is a deregulated isoform of plasma membrane Ca²⁺-ATPase of Arabidopsis thaliana.Plant molecular biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca(2)+-ATPases are key regulators of Ca(2+) ion efflux in all eukaryotes. Animal cells have two distinct families of Ca(2+) pumps, with calmodulin-stimulated pumps (type IIB pumps) found exclusively at the plasma membrane. In plants, no equivalent type IIB pump located at the plasma membrane has been identified at the molecular level, although related isoforms have been identified in non-plasma membrane locations. Here, we identify a plant cDNA, designated SCA1 (for soybean Ca(2+)-ATPase 1), that encodes Ca(2+)-ATPase and is located at the plasma membrane. The plasma membrane localization was determined by sucrose gradient and aqueous two-phase membrane fractionations and was confirmed by the localization of SCA1p tagged with a green fluorescent protein. The Ca(2+)-ATPase activity of the SCA1p was increased approximately sixfold by calmodulin (K(1/2) approximately 10 nM). Two calmodulin binding sequences were identified in the N-terminal domain. An N-terminal truncation mutant that deletes sequence through the two calmodulin binding sites was able to complement a yeast mutant (K616) that was deficient in two endogenous Ca(2+) pumps. Our results indicate that SCA1p is structurally distinct from the plasma membrane-localized Ca(2+) pump in animal cells, belonging instead to a novel family of plant type IIB pumps found in multiple subcellular locations. In plant cells from soybean, expression of this plasma membrane pump was highly and rapidly induced by salt (NaCl) stress and a fungal elicitor but not by osmotic stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.