ArticleInfection and immunity2000
Role of ribosomal protein L12 in gonococcal invasion of Hec1B cells.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 60 citations in OpenAlex.
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- Identification of a Ribosomal Protein RpsB as a Surface-Exposed Protein and Adhesin ofBioMed research international · 2019Article
- Comparison of Surface Proteomes of Adherence Variants of Listeria Monocytogenes Using LC-MS/MS for Identification of Potential Surface Adhesins.Pathogens (Basel, Switzerland) · 2016Article
- A 24-48 h fed Amblyomma americanum tick saliva immuno-proteome.BMC genomics · 2014Article
- Haemophilus influenzae porine ompP2 gene transfer mediated by graphene oxide nanoparticles with effects on transformation process and virulence bacterial capacity.Journal of nanobiotechnology · 2014Article
- The dam replacing gene product enhances Neisseria gonorrhoeae FA1090 viability and biofilm formation.Frontiers in microbiology · 2014Article
- Proteome analysis and serological characterization of surface-exposed proteins of Rickettsia heilongjiangensis.PloS one · 2013Article
- Erythrocytic stage-dependent regulation of oligomerization of Plasmodium ribosomal protein P2.The Journal of biological chemistry · 2012Article
- Surface proteome of "Mycobacterium avium subsp. hominissuis" during the early stages of macrophage infection.Infection and immunity · 2012Article
- A bacterial siren song: intimate interactions between Neisseria and neutrophils.Nature reviews. Microbiology · 2012Review
- Genomic and surface proteomic analysis of the canine pathogen Staphylococcus pseudintermedius reveals proteins that mediate adherence to the extracellular matrix.Infection and immunity · 2011Article
- Proteomics and transcriptomics characterization of bile stress response in probiotic Lactobacillus rhamnosus GG.Molecular & cellular proteomics : MCP · 2011Article
- Surfome analysis as a fast track to vaccine discovery: identification of a novel protective antigen for Group B Streptococcus hypervirulent strain COH1.Molecular & cellular proteomics : MCP · 2009Article
- Towards the immunoproteome of Neisseria meningitidis.PloS one · 2009Article
- Low-phosphate-dependent invasion resembles a general way for Neisseria gonorrhoeae to enter host cells.Infection and immunity · 2006Article
- Identification of Actinobacillus suis genes essential for the colonization of the upper respiratory tract of swine.Infection and immunity · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies led to the development of a model of contact-induced enhanced gonococcal invasion of human reproductive cells that utilizes the lutropin receptor (LHr) as both the induction signal for conversion to this enhanced-gonococcal-invasion phenotype (Inv(+) GC) and as the specific Inv(+) GC uptake mechanism. This model proposes that gonococci express a surface feature that mimics human chorionic gonadotropin (hCG), the cognate ligand for LHr, and that this structure is responsible for the specific and productive interaction of GC with LHr. In this report, we identify a 13-kDa gonococcal protein with immunological similarities to hCG. The antiserum reactivity is specific since interaction with the 13-kDa gonococcal protein can be blocked by the addition of highly purified hCG. This gonococcal "hCG-like" protein, purified from two-dimensional gels and by immunoprecipitation, was determined by N-terminal sequencing to be the ribosomal protein L12. We present evidence that gonococcal L12 is membrane associated and surface exposed in gonococci, as shown by immunoblot analysis of soluble and insoluble gonococcal protein and antibody adsorption studies with fixed GC. Using highly purified recombinant gonococcal L12, we show that preincubation of Inv(-) GC with micromolar amounts of rL12 leads to a subsequent five- to eightfold increase in invasion of the human endometrial cell line, Hec1B. In addition, nanomolar concentrations of exogenous L12 inhibits gonococcal invasion to approximately 70% of the level in controls. Thus, we propose a novel cellular location for the gonococcal ribosomal protein L12 and concomitant function in LHr-mediated gonococcal invasion of human reproductive cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.